@article { ISI:000312157100027,
	title = {In situ pH maintenance for mammalian cell cultures in shake flasks and tissue culture flasks},
	journal = {Biotechnology Progress},
	volume = {28},
	number = {6},
	year = {2012},
	month = {NOV},
	pages = {1605-1610},
	publisher = {WILEY-BLACKWELL},
	address = {111 RIVER ST, HOBOKEN 07030-5774, NJ USA},
	abstract = {<p>pH in animal cell cultures decreases due to production of metabolites like lactate. pH control via measurement and base addition is not easily possible in small-scale culture formats like tissue-culture flasks and shake flasks. A hydrogel-based system is reported for in situ pH maintenance without pH measurement in such formats, and is demonstrated to maintain pH between 6.8 and 7.2 for a suspension CHO cell line in CD CHO medium and between 7.3 and 7.5 for adherent A549 cells in DMEM:F12 containing 10\% FBS. This system for pH maintenance, along with our previous report of hydrogels for controlled nutrient delivery in shake flasks can allow shake flasks to better mimic bioreactor-based fed batch operation for initial screening during cell line and process development for recombinant protein production in mammalian cells. (C) 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012</p>},
	keywords = {high throughput screening, pH control for cell culture, pH control in shake flask, pH control in tissue culture flasks, small scale platforms for cell culture},
	issn = {8756-7938},
	doi = {10.1002/btpr.1614},
	author = {Pradhan, Ketaki and Pant, Tejal and Gadgil, Mugdha}
}
