@article { ISI:000325642800010,
	title = {Production, purification and characterization of cholesterol oxidase from a newly isolated streptomyces sp.},
	journal = {World Journal of Microbiology \& Biotechnology},
	volume = {29},
	number = {11},
	year = {2013},
	month = {NOV},
	pages = {2077-2085},
	publisher = {SPRINGER},
	address = {233 SPRING ST, NEW YORK, NY 10013 USA},
	abstract = {<p>Cholesterol oxidase production (COD) by a new isolate characterized as Streptomyces sp. was studied in different production media and fermentation conditions. Individual supplementation of 1 \% maltose, lactose, sucrose, peptone, soybean meal and yeast extract enhanced COD production by 80-110 \% in comparison to the basal production medium (2.4 U/ml). Supplementation of 0.05 \% cholesterol (inducer) enhanced COD production by 150 \%. COD was purified 14.3-fold and its molecular weight was found to be 62 kDa. V-max (21.93 mu M/min mg) and substrate affinity K-m (101.3 mu M) suggested high affinity of the COD for cholesterol. In presence of Ba2+ and Hg2+ the enzyme activity was inhibited while Cu2+ enhanced the activity nearly threefold. Relative activity of the enzyme was found maximum in triton X-100 whereas sodium dodecyl sulfate inactivated the enzyme. The enzyme activity was also inhibited by the thiol-reducing reagents like Dithiothreitol and beta-mercaptoethanol. The COD showed moderate stability towards all organic solvents except acetone, benzene and chloroform. The activity increased in presence of isopropanol and ethanol. The enzyme was most active at pH 7 and 37 A degrees C temperature. This organism is not reported to produce COD.</p>},
	keywords = {Affinity chromatography, Cholesterol oxidase, HPLC, SDS PAGE, Streptomyces sp.},
	issn = {0959-3993},
	doi = {10.1007/s11274-013-1371-8},
	author = {Niwas, Ram and Singh, Vineeta and Singh, Rajbir and Tripathi, Divya and Tripathi, C. K. M.}
}
