%0 Journal Article %J Bioorganic & Medicinal Chemistry Letters %D 2016 %T Azadirachta indica triterpenoids promote osteoblast differentiation and mineralization in vitro and in vivo %A Kushwaha, Priyanka %A Khedgikar, Vikram %A Haldar, Saikat %A Gautam, Jyoti %A Mulani, Fayaj A. %A Thulasiram, Hirekodathakallu V. %A Trivedi, Ritu %K ALP and mineralization assay %K Balb/c mice pups %K Calvarial osteoblast cells %K Gene expressions %X

Terpenoids were isolated using chromatographic purification through solvent purification technique and identified as Azadirone (1), Epoxyazadiradione (2) Azadiradione (3) Gedunin (4) Nimbin (5) Salannin (6) Azadirachtin A (7) and Azadirachtin B (8) from Azadirachta indica. Out of eight compounds, only three compounds had osteogenic activity and enhanced osteoblast proliferation, differentiation and mineralization in osteoblast cells. Active compounds stimulated osteogenic genes ALP, RunX-2 and OCN expressions in vitro, but Azadirachtin A had a maximum ability to stimulate osteoblast differentiation and mineralization compared to other two active compounds. For in vivo study, Azadirachtin A injected subcutaneously in pups, which enhanced osteogenic gene expressions and promoted bone formation rate significantly. Here, we conclude that active compounds of Azadirachta indica have osteogenic activity and Azadirachtin A has a beneficial effects on bone. (C) 2016 Elsevier Ltd. All rights reserved.

%B Bioorganic & Medicinal Chemistry Letters %I PERGAMON-ELSEVIER SCIENCE LTD %C THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND %V 26 %P 3719-3724 %8 AUG %G eng %N 15 %3

Foreign

%4 2.486 %R 10.1016/j.bmcl.2016.05.076