%0 Journal Article %J Journal of Biomedical Nanotechnology %D 2007 %T CdS quantum dots: enzyme mediated in vitro synthesis, characterization and conjugation with plant lectins %A Ansary, Abu Ayoobul %A Kumar, S. Anil %A Krishnasastry, M. V. %A Abyaneh, Majid Kazemian %A Kulkarni, Sulabha K. %A Ahmad, Absar %A Khan, Mohammad Islam %K bio-label %K CdS quantum dots %K conjugation %K Enzyme %K in vitro synthesis %K Nanoparticles %X

This paper presents the in vitro synthesis of CdS quantum dot nanoparticles using sulphite reductase and capping peptide in the presence of a co-factor alpha-NADPH, their characterization and conjugation with plant lectins, Jacalin and Chick pea lectin. Incubation of sulphite reductase with aqueous CdCl2, Na2SO3, and capping peptide in the presence of a-NADPH, which acts as a co-factor, resulted in the formation of CdS nanoparticles of dimensions 5-20 nm. These CdS nanoparticles were conjugated with plant lectins by EDAC mediated coupling. These observations are of significance, as it will help in the development of eco-friendly process for the formation of CdS nanoparticles and their application as fluorescent biolabels. Moreover the above studies will also help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.

%B Journal of Biomedical Nanotechnology %I AMER SCIENTIFIC PUBLISHERS %C 25650 NORTH LEWIS WAY, STEVENSON RANCH, CA 91381-1439 USA %V 3 %P 406-413 %8 DEC %G eng %N 4 %9 Article %3 Foreign %4 3.929 %R 10.1166/jbn.2007.045