%0 Journal Article %J Bioresource Technology %D 2010 %T Flocculation of dimorphic yeast benjaminiella poitrasii is altered by modulation of NAD-glutamate dehydrogenase %A Joshi, C. V. %A Ghormade, Vandana %A Kunde, P. %A Kulkarni, P. %A Mamgain, H. %A Bhat, Suresh K. %A Paknikar, K. M. %A Deshpande, Mukund V. %K Benjaminiella poitrasii %K dimorphism %K flocculation %K NAD-glutamate dehydrogenase %X

A strategy to control flocculation is investigated using dimorphic yeast, Benjaminiella poitrasii as a model. Parent form of this yeast (Y) exhibited faster flocculation (11.1 min) than the monomorphic yeast form mutant Y-5 (12.6 min). Atomic force microscopy revealed higher surface roughness of Y (439.34 rms) than Y-5 (52 rms). Also, the former had a zeta potential of -65.97 +/- 3.45 as against -50.21 +/- 2.49 for the latter. Flocculation of both Y and Y-5 could be altered by supplementing either substrates or inhibitor of NAD-glutamate dehydrogenase (NAD-GDH) in the growth media. The rate of flocculation was promoted by alpha-ketoglutarate or isophthalic acid and decelerated by glutamate with a statistically significant inverse correlation to corresponding NAD-GDH levels. These interesting findings open up new possibilities of using NAD-GDH modulating agents to control flocculation in fermentations for easier downstream processing. (C) 2009 Elsevier Ltd. All rights reserved.

%B Bioresource Technology %I ELSEVIER SCI LTD %C THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND %V 101 %P 1393-1395 %8 FEB %G eng %N 4 %3 Foreign %4 4.365 %R 10.1016/j.biortech.2009.09.052