%0 Journal Article %J Biotechnology Letters %D 2010 %T Strain improvement of lactobacillus lactis for d-lactic acid production %A Joshi, D. S. %A Singhvi, M. S. %A Khire, Jayant Malhar %A Gokhale, D. V. %K Cellobiose utilization %K D-Lactic acid production %K Lactobacillus lactis %K Mutant %K Sucrose (cane sugar) %X

Three mutants, isolated by repeated UV mutagenesis of Lactobacillus lactis NCIM 2368, produced increased d-lactic acid concentrations. These mutants were compared with the wild type using 100 g hydrolyzed cane sugar/l in the fermentation medium. One mutant, RM2-24, produced 81 g lactic acid/l which was over three times that of the wild type. The highest d-lactic acid (110 g/l) in batch fermentation was obtained with 150 g cane sugar/l with a 73% lactic acid yield. The mutant utilizes cellobiose efficiently, converting it into d-lactic acid suggesting the presence of cellobiase. Thus, this strain could be used to obtain d-lactic acid from cellulosic materials that are pre-hydrolyzed with cellulase.

%B Biotechnology Letters %I SPRINGER %C VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS %V 32 %P 517-520 %8 APR %G eng %N 4 %3 Foreign %4 1.768 %R 10.1007/s10529-009-0187-y