TY - JOUR T1 - Conserved RNA binding activity of phosphatidyl inositol 5-phosphate 4-kinase (PIP4K2A) JF - Frontiers in Molecular Biosciences Y1 - 2021 A1 - Behari, Jatin A1 - Borkar, Pranita A1 - Vindu, Arya A1 - Dandewad, Vishal A1 - Upadrasta, Sindhuri A1 - Shanmugam, Dhanasekaran A1 - Seshadri, Vasudevan KW - malaria KW - posttranscriptional gene regulation KW - RNA-protein interaction KW - translation regulation AB - Plasmodium falciparum is a causative agent for malaria and has a complex life cycle in human and mosquito hosts. During its life cycle, the malarial parasite Plasmodium goes through different asexual and sexual stages, in humans and mosquitoes. Expression of stage-specific proteins is important for successful completion of its life cycle and requires tight gene regulation. In the case of Plasmodium, due to relative paucity of the transcription factors, it is postulated that posttranscriptional regulation plays an important role in stage-specific gene expression. Translation repression of specific set of mRNA has been reported in gametocyte stages of the parasite. A conserved element present in the 3 ` UTR of some of these transcripts was identified. Phosphatidylinositol 5-phosphate 4-kinase (PIP4K2A) was identified as the protein that associates with these RNA. We now show that the RNA binding activity of PIP4K2A is independent of its kinase activity. We also observe that PIP4K2A is imported into the parasite from the host on Plasmodium berghei and Toxoplasma gondii. The RNA binding activity of PIP4K2A seems to be conserved across species from Drosophila and C. elegans to humans, suggesting that the RNA binding activity of PIP4K may be important, and there may be host transcripts that may be regulated by PIP4K2A. These results identify a novel RNA binding role for PIP4K2A that may not only play a role in Plasmodium propagation but may also function in regulating gene expression in multicellular organisms. VL - 8 U3 - Foreign U4 - 5.246 ER -