TY - JOUR T1 - ChIP-Seq data analysis: identification of protein-DNA binding sites with SISSRs peak-finder JF - Methods in molecular biology Y1 - 2012 A1 - Narlikar, Leelavati A1 - Jothi, Raja AB - Protein-DNA interactions play key roles in determining gene-expression programs during cellular development and differentiation. Chromatin immunoprecipitation (ChIP) is the most widely used assay for probing such interactions. With recent advances in sequencing technology, ChIP-Seq, an approach that combines ChIP and next-generation parallel sequencing is fast becoming the method of choice for mapping protein-DNA interactions on a genome-wide scale. Here, we briefly review the ChIP-Seq approach for mapping protein-DNA interactions and describe the use of the SISSRs peak-finder, a software tool for precise identification of protein-DNA binding sites from sequencing data generated using ChIP-Seq. VL - 802 U3 - Foreign U4 - 1.69 ER -