TY - JOUR T1 - Immobilization of invertase on functionalized mesoporous silica and properties of the immobilized biocatalyst JF - Research Journal of Biotechnology Y1 - 2007 A1 - Prabhune, Asmita Ashutosh A1 - Sulabha, Karandikar A1 - Archana, Pundle A1 - Shilpa, Deshpande A1 - Sanjeevani, Pardhy KW - beta fructofuranosidase KW - extrudates KW - functionalized mesoporous silica KW - Invert syrup KW - invertase KW - SBA-15 AB -

Invertase, which catalyses the hydrolysis of sucrose, is one of the simplest commercial carbohydrases and is used for the production of invert syrup (mixture of glucose and fructose). Fructose is the sweetest natural sugar having beneficial medicinal effects and is emerging as an alternative sweetener to sucrose in the pharmaceutical industry. beta fructofuranosidase (E C 3.2.1.26) purified from Kluyveromyces marxianus NCYC 2675, a thermotolerant yeast, was immobilized on functionalized mesoporous silica SBA- 15. Attempts were made to immobilize invertase on functionalized silicalite and cross linked with glutaraldehyde so as to obtain a highly active and stable immobilized enzyme preparation suitable for commercial exploitation. Cross linked enzyme silica complex as well as enzyme silica complex showed very good temperature and pH stability. Extrudates of the mesoporous silica also showed similar results. Bound enzyme showed excellent stability to repeated use and retained 90% of its initial activity after ten cycles of reuse in batch mode.

PB - RESEARCH JOURNAL BIOTECHNOLOGY CY - SECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIA VL - 2 IS - 1 U3 -

Indian

U4 - 2.667 ER -