TY - JOUR T1 - Characterization of a new bacillus cereus ATUAVP1846 strain producing penicillin V acylase, and optimization of fermentation parameters JF - Annals of Microbiology Y1 - 2012 A1 - Sunder, Avinash Vellore A1 - Kumar, Atul A1 - Naik, Neha A1 - Pundle, Archana V. KW - 16S rRNA KW - Bacillus cereus KW - isolation KW - Ntn hydrolase KW - Optimization KW - Penicillin acylase AB -

Penicillin acylases are involved chiefly in the industrial production of semisynthetic penicillins, which remain the most widely used group of antibiotics. We have isolated a new bacterial strain ATUAVP1846 that produces penicillin V acylase (PVA). Phylogenetic analysis using 16S rRNA sequencing showed 99.37% homology with Bacillus cereus. Maximum PVA production was observed with B. cereus ATUAVP1846 at 30A degrees C, pH 7 after 24 h fermentation time under submerged conditions. Highest enzyme productivity was achieved using sucrose as carbon source, and tryptone and ammonium hydrogen phosphate as nitrogen sources. Minimal medium containing 0.4% glucose and 0.3% ammonium hydrogen phosphate was found to be optimal for maximum PVA production from B. cereus ATUAVP1846. The crude enzyme from B. cereus ATUAVP1846 was partially purified using ammonium sulfate fractionation and showed highest enzymatic activity in the hydrolysis of penicillin V at 40A degrees C and pH 6. The crude enzyme preparation also showed unique substrate specificity, preferring ampicillin and cephalexin over penicillin V.

PB - SPRINGER CY - 233 SPRING ST, NEW YORK, NY 10013 USA VL - 62 IS - 3 U3 - Foreign U4 - 1.549 ER -