TY - JOUR T1 - Comparison of polymer induced and solvent induced trypsin denaturation: the role of hydrophobicity JF - Colloids and Surfaces B-Biointerfaces Y1 - 2014 A1 - Jasti, Lakshmi Swarnalatha A1 - Fadnavis, Nitin W. A1 - Addepally, Uma A1 - Daniels, Siona A1 - Deokar, Sarika Babasaheb A1 - Ponrathnam, Surendra KW - Adsorption KW - Denaturation KW - Hydrophobicity KW - Polymer KW - Trypsin AB -

Trypsin adsorption from aqueous buffer by various copolymers of allyl glycidyl ether-ethylene glycol dimethacrylate (AGE-EGDM) copolymer with varying crosslink density increases with increasing crosslink density and the effect slowly wears off after reaching a plateau at 50% crosslink density. The copolymer with 25% crosslink density was reacted with different amines with alkyl/aryl side chains to obtain a series of copolymers with 1,2-amino alcohol functional groups and varying hydrophobicity. Trypsin binding capacity again increases with hydrophobicity of the reacting amine and a good correlation between logP(octanol) of the amine and protein binding is observed. The bound trypsin is denatured to the extent of 90% in spite of the presence of hydrophilic hydroxyl and amino groups. The behavior was comparable to that in mixtures of aqueous buffer and water-miscible organic co-solvents where the solvent concentration required to deactivate 50% of the enzyme (C-50) is dependent on logP(octanol) of the co-solvent. (C) 2014 Elsevier B.V. All rights reserved.

PB - ELSEVIER SCIENCE BV CY - PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS VL - 116 U3 - Foreign U4 - 3.902 ER -