TY - JOUR T1 - Blends of shellac as nanofiber formulations for wound healing JF - Journal of Bioactive and Compatible Polymers Y1 - 2015 A1 - Vannuruswamy, Garikapati A1 - Rathna, Gundloori Venkata Naga A1 - Gadgil, Bhagyashri Shashikant Thorat A1 - Gadad, Anand Panchakshari KW - drug release and mechanism KW - nanofibers KW - Shellac KW - thermoresponsive KW - tissue regeneration AB -

Shellac is being used in food, pharmaceutical, and agricultural industries. It is seldom used for biomedical applications due to its poor mechanical property and instability. We designed thermoresponsive shellac-based bioactive nanofiber mats that mimic extracellular matrix to extend their utility in wound healing. Various blend compositions of shellac, gelatin, and poly(N-isopropylacrylamide) enriched with a bioactive agent, nadifloxacin, were prepared and nanofiber mats were fabricated. The morphology of the nanofiber formation was influenced by the concentration of polymer, drug, and polymer blend composition. Polymer-drug interactions and thermal and crystalline properties of nanofiber mats were analyzed. The shellac/gelatin/poly(N-isopropylacrylamide) blend of composition 3%/7%/3% (w/v) was chosen to evaluate in vitro drug release. Release studies recorded slow, constant, and sustained release for 140h. The release kinetics and mechanism confirmed zero-order release with resultant r(2) values greater than 0.99, and the Korsmeyer-Peppas release exponent (n) was slightly higher than 0.8, which indicated that drug diffusion was anomalous or non-Fickian type and drug release followed diffusion involving chain stretching. Indirect cytotoxicity studies recorded insignificant toxicity against normal human fibroblast cells. In vivo studies demonstrated that drug-loaded nanofiber mats were more suitable for faster tissue regeneration as compared to nanofiber mats without drug and commercial nadifloxacin cream.

PB - SAGE PUBLICATIONS LTD CY - 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND VL - 30 IS - 5 U3 -

Foreign

U4 - 1.568 ER -