02678nas a2200325   4500008004100000022001400041245013000055210006900185260000900254300001400263490000700277520154600284653003601830653001301866653001101879653002701890653002201917653002201939653003301961100002301994700002402017700002202041700002002063700002902083700002502112700003702137700002802174700002402202856012602226       2018                        eng d  a0022-095700aMiRNA160 is associated with local defense and systemic acquired resistance against Phytophthora infestans infection in potato0 aMiRNA160 is associated with local defense and systemic acquired   cAPR   a2023-20360 v693 a<p>To combat pathogen infection, plants employ local defenses in infected sites and elicit systemic acquired resistance (SAR) in distant tissues. MicroRNAs have been shown to play a significant role in local defense, but their association with SAR is unknown. In addition, no such studies of the interaction between potato and Phytophthora infestans have been reported. We investigated the role of miR160 in local and SAR responses to P. infestans infection in potato. Expression analysis revealed induced levels of miR160 in both local and systemic leaves of infected wild-type plants. miR160 overexpression and knockdown plants exhibited increased susceptibility to infection, suggesting that miR160 levels equivalent to those of wild-type plants may be necessary for mounting local defense responses. Additionally, miR160 knockdown lines failed to elicit SAR, and grafting assays indicated that miR160 is required in both local and systemic leaves to trigger SAR. Consistently, SAR-associated signals and genes were dysregulated in miR160 knockdown lines. Furthermore, analysis of the expression of defense and auxin pathway genes and direct regulation of StGH3.6, a mediator of salicylic acid-auxin cross-talk, by the miR160 target StARF10 revealed the involvement of miR160 in antagonistic cross-talk between salicylic acid-mediated defense and auxin-mediated growth pathways. Overall, our study demonstrates that miR160 plays a crucial role in local defense and SAR responses during the interaction between potato and P. infestans.</p>10aAuxin-salicylic acid antagonism10amicroRNA10amiR16010aPhytophthora infestans10aSolanum chacoense10aSolanum tuberosum10asystemic acquired resistance1 aNatarajan, Bhavani1 aKalsi, Harpreet, S.1 aGodbole, Prajakta1 aMalankar, Nilam1 aThiagarayaselvam, Aarthy1 aSiddappa, Sundaresha1 aThulasiram, Hirekodathakallu, V.1 aChakrabarti, Swarup, K.1 aBanerjee, Anjan, K.  uhttp://library.ncl.res.in/content/mirna160-associated-local-defense-and-systemic-acquired-resistance-against-phytophthora02479nas a2200289   4500008004100000022001400041245014000055210006900195260000900264300001200273490000700285520151800292653002001810653001601830653000801846653001601854653003201870653002401902653001401926653002201940653001201962100001601974700002901990700002402019700002402043856012202067       2021                        eng d  a0022-095700aPolycomb group methyltransferase StE(z)2 and deposition of H3K27me3 and H3K4me3 regulate the expression of tuberization genes in potato0 aPolycomb group methyltransferase StEz2 and deposition of H3K27me  cFEB   a426-4440 v723 a<p>Polycomb repressive complex (PRC) group proteins regulate various developmental processes in plants by repressing target genes via H3K27 trimethylation, and they function antagonistically with H3K4 trimethylation mediated by Trithorax group proteins. Tuberization in potato has been widely studied, but the role of histone modifications in this process is unknown. Recently, we showed that overexpression of StMSI1, a PRC2 member, alters the expression of tuberization genes in potato. As MSI1 lacks histone-modification activity, we hypothesized that this altered expression could be caused by another PRC2 member, StE(z)2, a potential H3K27 methyltransferase in potato. Here, we demonstrate that a short-day photoperiod influences StE(z)2 expression in the leaves and stolons. StE(z)2 overexpression alters plant architecture and reduces tuber yield, whereas its knockdown enhances yield. ChIP-sequencing using stolons induced by short-days indicated that several genes related to tuberization and phytohormones, such as StBEL5/11/29, StSWEET11B, StGA2OX1, and StPIN1 carry H3K4me3 or H3K27me3 marks and/or are StE(z)2 targets. Interestingly, we observed that another important tuberization gene, StSP6A, is targeted by StE(z)2 in leaves and that it has increased deposition of H3K27me3 under long-day (non-induced) conditions compared to short days. Overall, our results show that StE(z)2 and deposition of H3K27me3 and/or H3K4me3 marks might regulate the expression of key tuberization genes in potato.</p>10aChIP-sequencing10aEpigenetics10aPcG10aphotoperiod10apolycomb repressive complex10apotato tuberization10ashort-day10aSolanum tuberosum10aStE(z)21 aKumar, Amit1 aKondhare, Kirtikumar, R.1 aMalankar, Nilam, N.1 aBanerjee, Anjan, K.  uhttp://library.ncl.res.in/content/polycomb-group-methyltransferase-stez2-and-deposition-h3k27me3-and-h3k4me3-regulate