01722nas a2200217 4500008004100000022001400041245008800055210006900143260007400212300001000286490000700296520091600303653001901219653002301238653002201261653003101283653002501314100002401339700002501363856011601388 2009 eng d a0141-549200aChromate reduction by PVA-alginate immobilized streptomyces griseus in a bioreactor0 aChromate reduction by PVAalginate immobilized streptomyces grise aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcJAN a71-760 v313 a

Microbial reduction of toxic Cr(6+) to the less toxic Cr(3+) is potentially a useful bioremediation process. Among the matrices tested for whole cell immobilization of an efficient chromate-reducing Streptomyces griseus strain, PVA-alginate was the most effective and was used for reduction of Cr(VI) in a bioreactor. Cr(6+) reduction efficiency decreased as Cr(6+) was increased from 2 to 12 mg l(-1) but increased with an increase in biomass concentration. However, increasing the flow rate from 2 to 8 ml h(-1) did not significantly affect Cr(6+) reduction. The reduction was faster in simulated effluent than in synthetic medium and complete removal of 8 mg Cr(6+) l(-1) from effluent and synthetic medium occurred in 2 and 12 h, respectively. Our results indicate that immobilized S. griseus cells could be applied for the large-scale bioremediation of chromate-containing effluents and wastewaters.

10aBioremediation10aChromate reduction10aImmobilized cells10aPolyvinyl alcohol-alginate10aStreptomyces griseus1 aPoopal, Ashwini, C.1 aLaxman, Ryali, Seeta uhttp://library.ncl.res.in/content/chromate-reduction-pva-alginate-immobilized-streptomyces-griseus-bioreactor-002235nas a2200265 4500008004100000022001400041245009600055210006900151260000800220300001600228490000800244520137200252653001901624653001901643653001701662653002101679653001601700100001401716700002201730700002301752700002401775700002501799700002301824856012201847 2019 eng d a0304-389400aNovel fatty alkene from marine bacteria: a thermo stable biosurfactant and its applications0 aNovel fatty alkene from marine bacteria a thermo stable biosurfa cDEC aUNSP 1208680 v3803 a

In this study, a novel thermo stable biosurfactants, 1-Pentanonacontene (C95H190) a fatty alkene and 3-Hydroxy16-methylheptadecanoic acid (C18H36O3) were isolated from a marine isolate SGD-AC-13. Biosurfactants were produced using 1% yeast extract in tap water as production medium at 24 h in flask and 12 h in bioreactor. Using 16S rRNA gene sequence (1515 bp) and BCL card (bioMerieux VITEK (R)), strain was identified as Bacillus sp. Crude biosurfactant reduced the surface tension of distilled water to 31.32 +/- 0.93 mN/m with CMC value of 0.3 mg/ml. Cell free supernatant showed excellent emulsification and oil displacement activity with stability up to 160 degrees C, pH 6-12 and 50 g/L NaCl conc. Biosurfactants were characterized using FTIR, TLC, HPLC LC-MS and NMR spectroscopy. Cell free supernatant reduced the contact angle of distilled water droplet from 117 degrees to 52.28 degrees and of 2% pesticide from 78.77 degrees to 73.42 degrees while 750 mu g/ml of crude biosurfactant reduced from 66.06 degrees to 56.33 degrees for 2% pesticide and recovered 35% ULO and 12% HWCO from the contaminated sand. To our best of knowledge, this is the first report of thermo stable fatty alkene as a biosurfactant and is structurally different from previously reported, with having potential application in agriculture, oil recovery and bioremediation.

10aBioremediation10aBiosurfactants10aFatty alkene10aThermo stability10aWettability1 aRam, Hari1 aSahu, Amit, Kumar1 aSaid, Madhukar, S.1 aBanpurkar, Arun, G.1 aGajbhiye, Jayant, M.1 aDastager, Syed, G. uhttp://library.ncl.res.in/content/novel-fatty-alkene-marine-bacteria-thermo-stable-biosurfactant-and-its-applications02622nas a2200229 4500008004100000022001400041245012800055210006900183260000800252300001100260490000800271520184300279653001302122653001902135653001102154653002002165653001602185100002302201700001802224700001902242856013102261 2021 eng d a0013-935100aFunctional metagenomic landscape of polluted river reveals potential genes involved in degradation of xenobiotic pollutants0 aFunctional metagenomic landscape of polluted river reveals poten cJAN a1103320 v1923 a

Rapid industrialization contributes substantially to xenobiotic pollutants in rivers. As a result, most of the rivers traversing urban settlements are in significantly deteriorated conditions. These pollutants are recalcitrant, requiring robust catabolic machinery for their complete transformation into bioavailable and non-toxic byproducts. Microbes are versatile dwellers that could adapt to such contaminants by using them as a source of nutrients during growth. However, efficient bioremediation requires an in-depth knowledge of microbial diversity and their metabolism related genes in the polluted niches. We employed MinION shotgun sequencing, to comprehend the biodegradation related genes and their function potential operating in the polluted urban riverine system of Western India. A vast number of catabolic genes were detected for the xenobiotic pollutants such as Benzoate, Nitrotoluene, Aminobenzoate, Drug metabolism, and Polycyclic Aromatic Hydrocarbons. Aerobic, and anaerobic catabolism genes, were mapped for their ability of degradation of xenobiotics. Interestingly, catabolism profiles of multiple aromatic compounds culminated into the Benzoate degradation pathway, suggesting it as a plausible central pathway for the autochthonous bacterial communities. Further mapping with RemeDB database, predicted plastic and dye degrading enzymes. Moreover, the diversity indices for the pollutant degrading enzymes suggested little variations (R-2 value of 18%) between the city and non-city (outskirts of city limits) riverine stretch indicating the impact of industrialization in the outskirts of the city stretch as well. Altogether, this study would serve as a preliminary baseline for future explorations concerning river cleaning programs and also exploiting such microbes for bioremediation applications.

10aBenzoate10aBioremediation10aMinION10aRiverine system10aXenobiotics1 aYadav, Rakeshkumar1 aRajput, Vinay1 aDharne, Mahesh uhttp://library.ncl.res.in/content/functional-metagenomic-landscape-polluted-river-reveals-potential-genes-involved-degradation