02366nas a2200217 4500008004100000022001400041245015500055210006900210260010200279300001200381490000800393520150000401653002301901653001201924653001101936653001301947653000901960100002501969700003001994856012402024 2009 eng d a0253-413400aImaging hydrogen oxidation activity of catalyst-coated perfluoro sulfonic acid-polymer electrolyte membranes using scanning electrochemical microscopy0 aImaging hydrogen oxidation activity of catalystcoated perfluoro  aC V RAMAN AVENUE, SADASHIVANAGAR, P B \#8005, BANGALORE 560 080, INDIAbINDIAN ACAD SCIENCEScSEP a719-7250 v1213 a

Scanning Electrochemical Microscopy (SECM) is a unique technique for studying fast heterogeneous kinetics and to map reactivity gradients along the surface of an electrocatalyst, especially when it involves multiple surface sites of varying reactivity. It combines the dual advantages offered by ultramicroelectrode (UME) voltammetry in terms of reduced ohmic drop and insignificant double layer charging contribution with the advantages of imaging by rastering the UME across an electro-active surface. In this work, we demonstrate these distinctive features of SECM in evaluating reactivity gradients on catalyst (Pt/C) coated NafionA (R) films towards hydrogen oxidation activity, a reaction of immense technological relevance. Imaging has been performed in the feedback mode by allowing H(2) evolution at the tip (25 A mu m Pt UME), which is reoxidized at the substrate electrode containing Pt/C-Nafion film. Interesting distribution in H(2) oxidation activity has been observed as a function of potential applied to the Pt/C-Nafion film. In addition, a plot of normalized tip current versus the substrate electrode potential indicates the effect of potential-induced reactivity change in the catalyst-coated membranes. The results of the present investigation are believed to be useful to H(2)/O(2) PEM fuel cells with respect to evaluating reactivity gradients of catalyst-coated polymer electrolyte membranes, which is important to rectify problems related to catalyst utilization.

10ahydrogen oxidation10aImaging10aNafion10aplatinum10aSECM1 aParthasarathy, Meera1 aPillai, Vijayamohanan, K. uhttp://library.ncl.res.in/content/imaging-hydrogen-oxidation-activity-catalyst-coated-perfluoro-sulfonic-acid-polymer-002135nas a2200313 4500008004100000022001400041245010700055210006900162260007400231300000800305490000700313520111700320653001401437653001201451653001701463653001601480653001001496653001201506653001701518100001601535700001501551700002101566700002001587700001601607700002401623700002501647700001901672856013001691 2012 eng d a1388-076400aQuantum dot bio-conjugate: as a western blot probe for highly sensitive detection of cellular proteins0 aQuantum dot bioconjugate as a western blot probe for highly sens aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcMAR a7320 v143 a

In the present study, we report a quantum dot (QD)-tailored western blot analysis for a sensitive, rapid and flexible detection of the nuclear and cytoplasmic proteins. Highly luminescent CdTe and (CdTe) ZnS QDs are synthesized by aqueous method. High resolution transmission electron microscopy, Raman spectroscopy, fourier transform infrared spectroscopy, fluorescence spectroscopy and X-ray diffraction are used to characterize the properties of the quantum dots. The QDs are functionalized with antibodies of prostate apoptosis response-4 (Par-4), poly(ADP-ribose) polymerases and beta actin to specifically bind with the proteins localized in the nucleus and cytoplasm of the cells, respectively. The QD-conjugated antibodies are used to overcome the limitations of conventional western blot technique. The sensitivity and rapidity of protein detection in QD-based approach is very high, with detection limits up to 10 pg of protein. In addition, these labels provide the capability of enhanced identification and localization of marker proteins in intact cells by confocal laser scanning microscopy.

10aDetection10aImaging10ananomedicine10aquantum dot10aRapid10asensors10aWestern blot1 aKale, Sonia1 aKale, Anup1 aGholap, Haribhau1 aRana, Abhimanyu1 aDesai, Rama1 aBanpurkar, Arun, G.1 aOgale, Satishchandra1 aShastry, Padma uhttp://library.ncl.res.in/content/quantum-dot-bio-conjugate-western-blot-probe-highly-sensitive-detection-cellular-proteins-003140nas a2200253 4500008004100000022001400041245011700055210006900172260000900241300001600250490000700266520227500273653002302548653001902571653002402590653001702614653001202631653002202643653001802665653002902683100002102712700002702733856012602760 2023 eng d a1944-824400aEffective distribution of gold nanorods in ordered thick mesoporous silica: a choice of noninvasive theranostics0 aEffective distribution of gold nanorods in ordered thick mesopor cOCT  a47615-476270 v153 a

Porous silica coated gold nanorod core-shell structures demonstrate a multifunctional role in bioimaging, drug delivery, and cancer therapeutics applications. Here, we address a new approach for effective distribution of gold nanorods (GNRs) in a mesoporous silica (MS) shell, viz., one nanorod in one silica particle (GMS). We have studied that silica coating presents major advantages for the better biocompatibility and stability of GNRs. In this study, two different thicknesses of silica shell over GNRs have been discussed as per the application's need; GNRs in thin silica (11 nm) are fit for phototherapy and bioimaging, whereas thick and porous silica (51 nm) coated gold nanorods are suitable for triggered drug delivery and theranostics. However, effective distribution of GNRs in ordered architecture of thick mesoporous silica (MS, more than 50 nm thickness) with high surface area (more than 1000 m(2)/g) is not well understood so far. Here, we present methodical investigations for uniform and highly ordered mesoporous silica coating over GNRs with tunable thickness (6 to 51 nm). Judicious identification and optimization of different reaction parameters like concentrations of silica precursor (TEOS, 1.85-43.9 mM), template (CTAB, 0.9-5.7 mM), effect of temperature, pH (8.6-10.8), stirring speed (100-400 rpm), and, most importantly, the mode of addition of TEOS with GNRs have been discussed. Studies with thick, porous silica coated GNRs simplify the highest ever reported surface area (1100 m(2)/g) and cargo capacity (57%) with better product yield (g/batch). First and foremost, we report a highly scalable (more than 500 mL) and rapid direct deposition of an ordered MS shell around GNRs. These engineered core-shell nanoparticles demonstrate X-ray contrast property, synergistic photothermal-chemotherapeutics, and imaging of tumor cell (96% cell death) due to released fluorescent anticancer drug molecules and photothermal effect (52 degree celsius) of embedded GNRs. A deeper insight into their influence on the architectural features and superior theranostics performances has been illustrated in detail. Hence, these findings indicate the potential impact of individual GMS for image guided combination therapeutics of cancer.

10aCombinationTherapy10aContrast Agent10aCore-ShellStructure10aGoldNanorods10aImaging10aMesoporous silica10aNanoparticles10aTheranosticsIntervention1 aPrasad, Rajendra1 aSelvaraj, Kaliaperumal uhttp://library.ncl.res.in/content/effective-distribution-gold-nanorods-ordered-thick-mesoporous-silica-choice-noninvasive