02180nas a2200253   4500008004100000022001400041245012300055210006900178260000800247300001100255490000800266520133300274653001301607653001301620653002201633653001901655653001801674653001501692100002301707700002001730700002701750700002001777856012901797       2022                        eng d  a0031-942200aCharacterized constituents of insect herbivore oral secretions and their influence on the regulation of plant defenses0 aCharacterized constituents of insect herbivore oral secretions a  cJAN  a1130080 v1933 aFor more than 350 million years, there have been ongoing dynamic interactions between plants and insects. In several cases, insects cause-specific feeding damage with ensuing herbivore-associated molecular patterns that invoke characteristic defense responses. During feeding on plant tissue, insects release oral secretions (OSs) containing a repertoire of molecules affecting plant defense (effectors). Some of these OS components might elicit a defense response to combat insect attacks (elicitors), while some might curb the plant defenses (suppressors). Few reports suggest that the synthesis and function of OS components might depend on the host plant and associated microorganisms. We review these intricate plant-insect interactions, during which there is a continuous exchange of molecules between plants and feeding insects along with the associated microorganisms. We further provide a list of commonly identified inducible plant produced defensive molecules released upon insect attack as well as in response to OS treatments of the plants. Thus, we describe how plants specialized and defense-related metabolism is modulated at innumerable phases by OS during plant-insect interactions. A molecular understanding of these complex interactions will provide a means to design eco-friendly crop protection strategies.10aEffector10aElicitor10aHerbivore insects10aOral secretion10aPlant defense10aSuppressor1 aKallure, Gopal, S.1 aKumari, Archana1 aShinde, Balkrishna, A.1 aGiri, Ashok, P.  uhttp://library.ncl.res.in/content/characterized-constituents-insect-herbivore-oral-secretions-and-their-influence-regulation02281nas a2200265   4500008004100000022001400041245014600055210006900201260000800270300001100278490000800289520143600297653002101733653001201754653001601766653001301782653001901795653001401814100002301828700002701851700002501878700002001903700002001923856007201943       2022                        eng d  a0168-945200aDietary influence on modulation of Helicoverpa armigera oral secretion composition leading to differential regulation of tomato plant defense0 aDietary influence on modulation of Helicoverpa armigera oral sec  cJAN  a1111200 v3143 aLittle is known about how different plant-based diets influence the insect herbivores’ oral secretion (OS) composition and eventually the plant defense responses. We analyzed the OS composition of the generalist Lepidopteran insect, Helicoverpa armigera feeding on the host plant tomato (OSH), non-host plant capsicum (OSNH), and artificial diet (OSAD) using Liquid Chromatography-Quadrupole Time of Flight Mass Spectrometry. Higher numbers and levels of alkaloids and terpenoids were observed in OSH and OSNH, respectively while OSAD was rich in phospholipids. Interestingly, treatment of H. armigera OSAD, OSH and OSNH on wounded tomato leaves showed differential expression of (i) genes involved in JA and SA biosynthesis and their responsive genes, and (ii) biosynthetic pathway genes of chlorogenic acid (CGA) and trehalose, which exhibited increased accumulation along with several other plant defensive metabolites. Specifically, high levels of CGA were detected after OSH and OSNH treatments in tomato leaves. There was higher expression of the genes involved in phenylpropanoid biosynthesis, which may lead to the increased accumulation of CGA and related metabolites. In the insect bioassay, CGA significantly inhibited H. armigera larval growth. Our results underline the differential accumulation of plant and insect OS metabolites and identified potential plant metabolite(s) affecting insect growth and development.10aChlorogenic acid10adefense10aMetabolites10aNon-host10aOral secretion10atrehalose1 aKallure, Gopal, S.1 aShinde, Balkrishna, A.1 aBarvkar, Vitthal, T.1 aKumari, Archana1 aGiri, Ashok, P.  uhttps://www.sciencedirect.com/science/article/pii/S016894522100316202346nas a2200265   4500008004100000022001400041245014700055210006900202260000800271300001100279490000800290520144700298653002101745653001201766653001601778653001301794653001901807653001401826100002301840700002701863700002501890700002001915700002001935856012501955       2022                        eng d  a0168-945200aDietary influence on modulation of Helicoverpa armigera oral secretion composition leading to differential regulation of tomato plant defense.0 aDietary influence on modulation of Helicoverpa armigera oral sec  cJAN  a1111200 v3143 a<p>
	Little is known about how different plant-based diets influence the insect herbivores' oral secretion (OS) composition and eventually the plant defense responses. We analyzed the OS composition of the generalist Lepidopteran insect, Helicoverpa armigera feeding on the host plant tomato (OSH), non-host plant capsicum (OSNH), and artificial diet (OSAD) using Liquid Chromatography-Quadrupole Time of Flight Mass Spectrometry. Higher numbers and levels of alkaloids and terpenoids were observed in OSH and OSNH, respectively while OSAD was rich in phospholipids. Interestingly, treatment of H. armigera OSAD, OSH and OSNH on wounded tomato leaves showed differential expression of (i) genes involved in JA and SA biosynthesis and their responsive genes, and (ii) biosynthetic pathway genes of chlorogenic acid (CGA) and trehalose, which exhibited increased accumulation along with several other plant defensive metabolites. Specifically, high levels of CGA were detected after OSH and OSNH treatments in tomato leaves. There was higher expression of the genes involved in phenyl-propanoid biosynthesis, which may lead to the increased accumulation of CGA and related metabolites. In the insect bioassay, CGA significantly inhibited H. armigera larval growth. Our results underline the differential accumulation of plant and insect OS metabolites and identified potential plant metabolite(s) affecting insect growth and development.</p>
10aChlorogenic acid10adefense10aMetabolites10aNon-host10aOral secretion10atrehalose1 aKallure, Gopal, S.1 aShinde, Balkrishna, A.1 aBarvkar, Vitthal, T.1 aKumari, Archana1 aGiri, Ashok, P.  uhttp://library.ncl.res.in/content/dietary-influence-modulation-helicoverpa-armigera-oral-secretion-composition-leading-002321nas a2200253   4500008004100000022001400041245014300055210006900198260000800267490000800275520140100283653001701684653003701701653001401738653002501752653001901777100002301796700003201819700002101851700002501872700002201897700002001919856012801939       2024                        eng d  a0965-174800aAminoacylase efficiently hydrolyses fatty acid amino acid conjugates of Helicoverpa armigera potentially to increase the pool of glutamine0 aAminoacylase efficiently hydrolyses fatty acid amino acid conjug  cFEB0 v1653 a<p>
	One of the most prevalent bioactive molecules present in the oral secretion (OS) of lepidopteran insects is fatty acid amino acid conjugates (FACs). Insect dietary components have influence on the synthesis and retaining the pool of FACs in the OS. We noted differential and diet-specific accumulation of FACs in the OS of Helicoverpa armigera by using Liquid Chromatography-Quadrupole Time of Flight Mass Spectrometry. Interestingly, we identified FACs hydrolyzing enzyme aminoacylase (HaACY) in the OS of H. armigera through proteomic analysis. Next, we have cloned, expressed, and purified active recombinant HaACY in the bacterial system. Recombinant HaACY hydrolyzes all the six identified FACs in the OS of H. armigera larvae fed on host and non-host plants and releases respective fatty acid and glutamine. In these six FACs, fatty acid moieties vary while amino acid glutamine was common. Glutamine obtained upon hydrolysis of FACs by HaACY might serve as an amino acid pool for insect growth and development. To understand the substrate choices of HaACY, we chemically synthesized, purified, and characterized all the six FACs. Interestingly, rHaACY also shows hydrolysis of synthetic FACs into respective fatty acid and glutamine. Our results underline the importance of diet on accumulation of FACs and role of aminoacylase(s) in regulating the level of FACs and glutamine.</p>
10aAminoacylase10aFatty acid amino acid conjugates10aGlutamine10aHelicoverpa armigera10aOral secretion1 aKallure, Gopal, S.1 aSahoo, Shubhranshu, Shekhar1 aKale, Rutuja, S.1 aBarvkar, Vitthal, T.1 aKontham, Ravindar1 aGiri, Ashok, P.  uhttp://library.ncl.res.in/content/aminoacylase-efficiently-hydrolyses-fatty-acid-amino-acid-conjugates-helicoverpa-armigera