02063nas a2200265   4500008004100000022001400041245011200055210006900167260000800236300001400244490000700258520120900265653000601474653001701480653001701497653002101514653001301535653001301548653002701561100001801588700002001606700001901626700002501645856012701670       2021                        eng d  a0268-257500aBioglycerol (C3) upgrading to 2,3-butanediol (C4) by cell-free extracts of Enterobacter aerogenes NCIM 26950 aBioglycerol C3 upgrading to 23butanediol C4 by cellfree extracts  cMAY  a1316-13250 v963 a<p>BACKGROUND Production of biobased chemicals from renewable resources is a green starring approach that serves as a substitute to petroleum derivatives. Bioglycerol, with its growing production as a co-product of biodiesel, is an attractive low-cost feedstock for the synthesis of platform chemicals by microbial fermentation. 2,3-butanediol (2,3-BDO) is amongst the top biorefinery platform chemicals that can be produced by glycerol fermentation. RESULTS The `Circular Economy' concept is demonstrated by converting the by-product bioglycerol using a cell-free extract of Enterobacter aerogenes NCIM 2695 (National Collection of Industrial Microorganisms, NCIM), yielding 22 g L-1 2,3-BDO, in 96 h, 98% atom economy and 0.4 g/g E factor. The cell-free bioglycerol conversion to 2,3-BDO was proved using a modified procedure for determining glycerol dehydrogenase enzyme assay by protein analysis and it was also shown to be cell-bounded. CONCLUSION Our study offers an effective utilization of the leftover material (i.e. cell-free extract) that biocatalysed C3 to C4 diol, which adds value to the overall economics of the process using only crude glycerol (C3) itself as a fermentative medium.</p>
10a210a3-Butanediol10aatom economy10acircular economy10aE factor10aGlycerol10aglycerol dehydrogenase1 aParate, Roopa1 aBorgave, Mrunal1 aDharne, Mahesh1 aRode, Chandrashekhar  uhttp://library.ncl.res.in/content/bioglycerol-c3-upgrading-23-butanediol-c4-cell-free-extracts-enterobacter-aerogenes-ncim02334nas a2200217   4500008004100000022001400041245019500055210006900250260000800319300001100327490000800338520148600346653001701832653002101849653002901870653001601899653002301915100002101938700002301959856013401982       2023                        eng d  a0926-669000aEffective valorization of blackstrap molasses to poly gamma glutamic acid (γ-PGA) using L-glutamic acid independent feeding approach and its significance as drought mitigator in wheat plant0 aEffective valorization of blackstrap molasses to poly gamma glut  cOCT  a1169850 v2023 a<p>
	One of the finest approaches towards sustainable waste management is its effective valorization to value-added products (VAPs). Waste valorization is garnering considerable attention since it delivers substantial advantages, including environmental stability and a sustainable and viable platform for producing different VAPs. One such VAP is the poly gamma glutamic acid (&amp; gamma;- PGA), an extortionate biopolymer known to date. Its exorbitant cost is the main barrier preventing &amp; gamma;- PGA from becoming commercially viable. Hence, the key to the successful commercialization of &amp; gamma;- PGA is its sustainable and cost-effective production. In the present study, the ability of Bacillus paralicheniformis NCIM 5769 to utilize molasses for &amp; gamma;-PGA production with minimal nutrients was investigated. Results showed that 30% molasses combined with 2% ammonium nitrate was the optimal medium for &amp; gamma;-PGA production. Additionally, the anti-drought activity of the synthesized &amp; gamma;-PGA was evaluated on wheat plants showing significantly higher shoot length, biomass content, and germination rate compared to the control group. These findings suggest that &amp; gamma;-PGA produced from molasses has the potential to be used as a drought mitigator in wheat plant, thereby achieving the mission of circular economy and sustainable development goal (SGD) 12, i.e. pursuing sustainability patterns for consumption &amp; production.</p>
10aAnti-drought10acircular economy10aPoly gamma glutamic acid10aSustainable10aWaste valorization1 aNair, Pranav, G.1 aDharne, Mahesh, S.  uhttp://library.ncl.res.in/content/effective-valorization-blackstrap-molasses-poly-gamma-glutamic-acid-%CE%B3-pga-using-l-glutamic