02275nas a2200229   4500008004100000022001400041245011400055210006900169260000800238300000800246490000700254520148700261653001901748653002201767653002401789653003301813653001101846100001901857700002001876700002101896856012801917       2020                        eng d  a2190-572X00aCharacterization of multidrug resistance in Vibriospecies isolated from marine invertebrates from Andaman Sea0 aCharacterization of multidrug resistance in Vibriospecies isolat  cSEP  a4560 v103 a<p>This study describes the abundance of multidrug-resistantVibriosassociated with marine invertebrate hosts from the Andaman Sea, India. Thirty-eightVibriostrains were isolated from surface mucus layers of coralPorites,Goniastrea, Pocillopora, Fungia, and eggs of spiny lobster (Panulirus penicillatus). Phenotypically, the majority of strains exhibited growth at a wide range of temperatures, salt tolerance, and diverse nutritional requirements. All the strains had more than 97% 16S rRNA sequence similarity with type species of the genusVibriowhereVibrio fortis, andVibrio alginolyticuswere predominant. Multilocus Sequence Analysis (MLSA) using eight housekeeping genes namelyftsZ,gapA,gyrB,mreB,pyrH,recA,rpoA,andtopAdistributed the strains into 6 reported clades i.e.,Harveyi, Ponticus, Nereis, Orientalis, Splendidus,andMediterraneiwhere nearly half of the total strains represented the cladeHarveyi, followed by the cladeSplendidus.Likewise, the PFGE profile indicated genomic heterogeneity among the strains resulting in their distribution in five major clusters. Resistance to different antimicrobials was tested following the disc diffusion method where all strains were found susceptible to chloramphenicol (30 mu g) and resistant to streptomycin (10 mu g), vancomycin (30 mu g), sulfamethoxazole-trimethoprim (25 mu g). Moreover, the resistant phenotype to other antimicrobials confirmed the abundance of multidrug resistance strains in this marine environment.</p>
10aDrug-resistant10aGenetic diversity10amarine invertebrate10aMultilocus sequence analysis10aVibrio1 aKumari, Prabla1 aPoddar, Abhijit1 aDas, Subrata, K.  uhttp://library.ncl.res.in/content/characterization-multidrug-resistance-vibriospecies-isolated-marine-invertebrates-andaman