02771nas a2200241 4500008004100000022001400041245012800055210006900183260007300252300001200325490000800337520181200345653003102157653002202188653003702210653003102247653002402278100002402302700002502326700002302351700002602374856012902400 2014 eng d a0378-517300aStructure function attributes of gold nanoparticle vaccine association: effect of particle size and association temperature0 aStructure function attributes of gold nanoparticle vaccine assoc aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcAUG a439-4480 v4713 a

Many biotherapeutic applications of gold nanoparticles make use of conjugated or adsorbed protein moieties. Physical parameters of association such as particle size, morphology, surface chemistry and temperature influences the protein-nanoparticle association and thereby their interaction with the biological environment. In present study, effect of size of chitosan reduced gold nanoparticles (CsAuNPs) and association temperature on structure and function of tetanus toxoid (TT) vaccine has been investigated. CsAuNPs were synthesized in the sizes of 20 + 3, 40 + 5 and 80 + 7 nm followed by loading of TT. Binding process of CsAuNPs with TT was investigated at their predetermined micro molar concentrations. Upon binding of TT onto CsAuNPs, particle surface was characterized using X-ray photoelectron spectroscopy. CD spectroscopic evaluation of TT bound 20 nm CsAuNPs led to 75% reduction in secondary structure of TT and thereby compromised immune function. Binding of TT with 40 and 80 nm sized CsAuNPs did not cause significant modifications in secondary structure or function of TT. Thermodynamic studies using temperature dependent fluorescence spectroscopy revealed an increase in association constants with the temperature. Based on thermodynamic data three phases in CsAuNPs and TT association process were traced. Samples from these distinct phases were also investigated for immunological recognition. Ex-vivo interaction of TT-CsAuNPs with TT positive and negative sera followed by relative change in particle size and zeta potential was studied. The findings here suggests prominent role of particle size and association temperature on adsorbed TT structure and function. Such studies may help in engineering functional nanotherapeutics. (C) 2014 Elsevier B.V. All rights reserved.

10aAssociation thermodynamics10aGold nanoparticle10aNanoparticle protein interaction10aStructure function studies10aVaccine formulation1 aBarhate, Ganesh, A.1 aGaikwad, Sushama, M.1 aJadhav, Suresh, S.1 aPokharkar, Varsha, B. uhttp://library.ncl.res.in/content/structure-function-attributes-gold-nanoparticle-vaccine-association-effect-particle-size-002118nas a2200253 4500008004100000022001400041245008000055210006900135260010600204300001400310490000700324520123900331653002201570653002401592653001801616653001901634653000901653100001901662700002401681700001801705700001801723700001801741856010501759 2016 eng d a1364-032100aTiO2 modification by gold (Au) for photocatalytic hydrogen (H-2) production0 aTiO2 modification by gold Au for photocatalytic hydrogen H2 prod aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcMAY a1366-13750 v583 a

TiO2 is indeed one of the widely used semiconductors employed for photocatalytic hydrogen production. Most of its photocatalytic activity-is achieved in its crystalline form. However, its photocatalytic activity is limited to ultraviolet region. For making TiO2 visible light active; Au deposition is strongly recommended due to its surface plasmon feature. Au deposition enhances the photocatalytic activity of both crystalline and nanocrystalline TiO2. Efficiency of photocatalytic activity is controlled by shape and size of Au nanoparticle subsequently the synthetic methodology plays an important role. Herein, we furnish a brief description of TiO2-Au nanocomposite synthesis by different methods viz. sol-gel, photodeposition, deposition-precipitation simple reducing method and dispersion method. A discussion on physical properties of the resultant material is also provided. Three different types of mechanism has been described depending on the type of irradiation and form of TiO2 (crystalline and nanocrytalline). A comparative hydrogen production yield is also tabulated to get an idea about the best synthesis methodology and form of TiO2 for efficient photocatalysis. (C) 2016 Elsevier Ltd. All rights reserved.

10aGold nanoparticle10aHydrogen generation10aNanocomposite10aPhotocatalysis10aTiO21 aGupta, Bhavana1 aMelvin, Ambrose, A.1 aMatthews, Tom1 aDash, Sitaram1 aTyagi, A., K. uhttp://library.ncl.res.in/content/tio2-modification-gold-au-photocatalytic-hydrogen-h-2-production-002386nas a2200253 4500008004100000022001400041245013900055210006900194260000900263300001400272490000600286520150800292653001801800653001501818653002101833653002101854653002201875653002601897653002601923100002301949700002301972700001901995856011802014 2019 eng d a2365-654900aAdvances in the experimental and theoretical understandings of antibiotic conjugated gold nanoparticles for antibacterial applications0 aAdvances in the experimental and theoretical understandings of a cJUN  a6719-67380 v43 a

The worldwide bacterial resistance to a wide range of antibiotics originates a global health concern and calls for the development of new antibacterial agents. Over the recent years, nanomaterials-based agents have been proven to be useful for the effective antibacterial applications. Notably, the gold nanoparticles (AuNPs) draw particular attention due to their biological inertness and easy surface functionalization. It is now established that the antibiotic functionalization on the AuNPs surfaces increases the antibacterial efficacy even towards the antibiotic-resistant bacterial cells. Moreover, the antibacterial efficacy can be further enhanced by photothermal therapy using antibiotic conjugated AuNPs. In this review article, we have reviewed the advances in the development of the synthesis methods of antibiotic conjugated AuNPs and gold nanoclusters, and their antibacterial efficacy. We have also discussed the developments in the theoretical understandings behind the interaction of antibiotic molecules with gold surface and its relation to the antibacterial activity. We believe that few parameters including the selection of antibiotic molecules, the method of its attachment to AuNPs, the purification of antibiotic conjugated AuNPs, and the quantification of conjugated antibiotic are crucial and needs to be properly addressed. Moreover, there are many other future directions discussed, for using antibiotic conjugated AuNPs more effectively for antibacterial therapy.

10aAntibacterial10aantibiotic10aDFT calculations10agold nanocluster10aGold nanoparticle10amulti-drug resistance10aphoto-thermal therapy1 aKhandelwal, Puneet1 aSingh, Dheeraj, K.1 aPoddar, Pankaj uhttp://library.ncl.res.in/content/advances-experimental-and-theoretical-understandings-antibiotic-conjugated-gold02445nas a2200277 4500008004100000022001400041245017700055210006900232260000800301300001100309490000900320520145300329653001601782653002201798653002201820653002101842653002101863100002801884700001901912700001801931700001801949700002501967700001901992700002702011856012902038 2023 eng d a0022-286000aInteractive study of Au-20 nanocluster and methyl substituted amide linked tyrosine/tryptophan to develop representative model for studying protein-nanoparticle interaction0 aInteractive study of Au20 nanocluster and methyl substituted ami cJAN a1341770 v12723 a

Investigations on UV-vis, steady-state fluorescence and synchronous fluorescence properties of human hemoglobin (HHb) reveals greater spectral shifts in peaks arising due to alterations in microenvironment of Tyr-compared to Trp-during interactions with gold nanoparticle (GNP). Besides, interactions of Au-20 nanocluster with modified Tyr/Trp (possessing methyl substituted amide bonds) by quantum chemical calculations demonstrate significant role of amide bonds and the spectral shift, binding energy and alteration in bond distances appear to be higher for modified Tyr-compared to modified Trp. Moreover, close resemblance of frequency shift of modified Tyr/Trp-in presence of Au 20 is observed with respect to the experimental FT-IR study of HHb upon interaction with GNP, suggesting participation of amide bonds in both cases. Furthermore, CD DFT calculations using optimized helical stretch of HHbin presence of Au-20 and experimental CD results of HHb in presence of GNP further indicate participation amide bonds and biocompatibility of GNP. Apparently, the theoretical and experimental interactions are going in good agreement with each other. Overall, the study of interactions of modified Tyr/Trp-as representative models of protein microenvironment and Au-20 nanocluster as prototype of GNP to develop models for exploring protein nanoparticle interactions has been highlighted. (C) 2022 Elsevier B.V. All rights reserved.

10aamide bonds10aAu-20 nanocluster10aGold nanoparticle10aHuman hemoglobin10aModified Tyr/Trp1 aBanerjee, Nineesha, Sen1 aGhosh, Deborin1 aMitra, Ishani1 aPaul, Somnath1 aShow, Bibhutibhushan1 aGanguly, Tapan1 aChakraborty, Madhurima uhttp://library.ncl.res.in/content/interactive-study-au-20-nanocluster-and-methyl-substituted-amide-linked-tyrosinetryptophan