02772nas a2200241   4500008004100000022001400041245011700055210006900172260007100241300001600312490000800328520190000336100001502236700001702251700001902268700001902287700002202306700001502328700002302343700002502366700001702391856012202408       2015                        eng d  a1520-610600aSpecific reagent for Cr(III): imaging cellular uptake of Cr(III) in Hct116 cells and theoretical rationalization0 aSpecific reagent for CrIII imaging cellular uptake of CrIII in H  a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcOCT  a13018-130260 v1193 a<p>A new rhodamine-based reagent (L-1), trapped inside the micellar structure of biologically benign Triton-X 100, could be used for specific recognition of Cr(III) in aqueous buffer medium having physiological pH. This visible light excitable reagent on selective binding to Cr(III) resulted in a strong fluorescence turn-on response with a maximum at similar to 583 nm and tail of that luminescence band extended until 650 nm, an optical response that is desired for avoiding the cellular autofluorescence. Interference studies confirm that other metal ions do not interfere with the detection process of Cr(III) in aqueous buffer medium having pH 7.2. To examine the nature of binding of Cr(III) to L-1, various spectroscopic studies are performed with the model reagent L-1 which tend to support Cr(III)-eta(2)-olefin pi-interactions involving two olefin bonds in molecular probe L-1. Computational studies are also performed with another model reagent L-M to examine the possibility of such Cr(III)-eta(2)-olefin pi-interactions. Presumably, polar functional groups of the model reagent L-M upon coordination to the Cr(III) center effectively reduce the formal charge on the metal ion and this is further substantiated by results of the theoretical studies. This assembly is found to be cell membrane permeable and shows insignificant toxicity toward live colon cancer cells (Hct116). Confocal laser scanning microscopic studies further revealed that the reagent L-1 could be used as an imaging reagent for detection of cellular uptake of Cr(III) in pure aqueous buffer medium by Hct116 cells. Examples of a specific reagent for paramagnetic Cr(III) with luminescence ON response are scanty in the contemporary literature. This ligand design helped us in achieving the turn on response by utilizing the conversion from spirolactam to an acyclic xanthene form on coordination to Cr(III).</p>1 aAli, Firoj1 aSaha, Sukdeb1 aMaity, Arunava1 aTaye, Nandaraj1 aSi, Mrinal, Kanti1 aSuresh, E.1 aGanguly, Bishwajit1 aChattopadhyay, Samit1 aDas, Amitava  uhttp://library.ncl.res.in/content/specific-reagent-criii-imaging-cellular-uptake-criii-hct116-cells-and-theoretical-001329nas a2200205   4500008004100000022001400041245012700055210006900182260011000251300001400361490000700375520049600382100001500878700001800893700001900911700002300930700002500953700001700978856012800995       2016                        eng d  a1359-734500aSpecific receptor for hydrazine: mapping the in situ release of hydrazine in live cells and in an in vitro enzymatic assay0 aSpecific receptor for hydrazine mapping the in situ release of h  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcMAR  a6166-61690 v523 a<p>We report a new chemodosimetric reagent capable of detecting hydrazine in the presence of several other competing amine derivatives and ionic analytes of biological relevance. This reagent has been utilized for real time monitoring of in situ N2H4 release during the metabolism of a crucial tuberculosis drug, isoniazid, in live HepG2 cells. The fluorescence response of the reagent based on its specific reaction with N2H4 is used for developing an in vitro assay for aminoacylase-1.</p>1 aAli, Firoj1 aAnila, H., A.1 aTaye, Nandaraj1 aMogare, Devraj, G.1 aChattopadhyay, Samit1 aDas, Amitava  uhttp://library.ncl.res.in/content/specific-receptor-hydrazine-mapping-situ-release-hydrazine-live-cells-and-vitro-enzymatic01411nas a2200205   4500008004100000245008700041210006900128260000800197300001600205490000700221520072100228100001500949700002500964700002700989700002001016700002401036700002001060700001701080856010801097       2017                        eng d00aSuper-resolution probe to monitor HNO levels in the endoplasmic reticulum of cells0 aSuperresolution probe to monitor HNO levels in the endoplasmic r  cNOV  a12087-120930 v893 aSelective detection of nitroxyl (HNO), which has recently been identified as a reactive nitrogen species, is a challenging task. We report a BODIPY-based luminescence ON reagent for detection of HNO in aqueous solution and in live RAW 264.7 cells, based on the soft nucleophilicity of the phosphine oxide functionality toward HNO. The probe shows high selectivity to HNO over other reactive oxygen/nitrogen and sulfur species. Luminescence properties of the BODIPY-based chemodosimetric reagent make it an ideal candidate for use as a reagent for super-resolution structured illumination microscopy. The viability of the reagent for biological in vivo imaging application was also confirmed using Artemia as a model.1 aAli, Firoj1 aSreedharan, Sreejesh1 aAshoka, Anila, Hoskere1 aSaeed, Hiwa, K.1 aSmythe, Carl, G. W.1 aThomas, Jim, A.1 aDas, Amitava  uhttp://library.ncl.res.in/content/super-resolution-probe-monitor-hno-levels-endoplasmic-reticulum-cells