02627nas a2200229 4500008004100000022001400041245016400055210006900219260008000288300001400368490000700382520169500389653002202084653001202106653003402118653002902152653002402181100002402205700001602229700002602245856012602271 2011 eng d a1367-543500aHigh level phytase production by aspergillus niger NCIM 563 in solid state culture: response surface optimization, up-scaling, and its partial characterization0 aHigh level phytase production by aspergillus niger NCIM 563 in s aTIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANYbSPRINGER HEIDELBERGcSEP a1407-14170 v383 a

Phytase production by Aspergillus niger NCIM 563 was optimized by using wheat bran in solid state fermentation (SSF). An integrated statistical optimization approach involving the combination of Placket-Burman design (PBD) and Box-Behnken design (BBD) was employed. PBD was used to evaluate the effect of 11 variables related to phytase production, and five statistically significant variables, namely, glucose, dextrin, NaNO(3), distilled water, and MgSO(4)center dot 7H(2)O, were selected for further optimization studies. The levels of five variables for maximum phytase production were determined by a BBD. Phytase production improved from 50 IU/g dry moldy bran (DMB) to 154 IU/g DMB indicating 3.08-fold increase after optimization. A simultaneous reduction in fermentation time from 7 to 4 days shows a high productivity of 38,500 IU/kg/day. Scaling up the process in trays gave reproducible phytase production overcoming industrial constraints of practicability and economics. The culture extract also had 133.2, 41.58, and 310.34 IU/g DMB of xylanase, cellulase, and amylase activities, respectively. The partially purified phytase was optimally active at 55A degrees C and pH 6.0. The enzyme retained ca. 75% activity over a wide pH range 2.0-9.5. It also released more inorganic phosphorus from soybean meal in a broad pH range from 2.5 to 6.5 under emulated gastric conditions. Molecular weight of phytase on Sephacryl S-200 was approximately 87 kDa. The K (m) and V (max) observed were 0.156 mM and 220 mu m/min/mg. The SSF phytase from A. niger NCIM 563 offers an economical production capability and its wide pH stability shows its suitability for use in poultry feed.

10aAspergillus niger10aPhytase10aResponse surface optimization10aSolid state fermentation10aStatistical methods1 aBhavsar, Kavita, P.1 aKumar, Ravi1 aKhire, Jayant, Malhar uhttp://library.ncl.res.in/content/high-level-phytase-production-aspergillus-niger-ncim-563-solid-state-culture-response-002145nas a2200229 4500008004100000022001400041245018200055210006900237260009900306300001400405490000700419520119100426653002201617653002901639653001201668653002401680653001701704100002401721700001601745700002601761856012801787 2012 eng d a1359-511300aDownstream processing of extracellular phytase from aspergillus niger: chromatography process vs. aqueous two phase extraction for its simultaneous partitioning and purification0 aDownstream processing of extracellular phytase from aspergillus  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcJUL a1066-10720 v473 a

The application of single step aqueous two-phase extraction (ATPE) for the downstream processing of phytase from Aspergillus niger NCIM 563, produced under solid state fermentation, has been studied and compared with the traditional multi-step procedure involving salt precipitation and column chromatography. High phytase recovery (98.5%) within a short time (3 h) and improved thermostability was attained by ATPE in comparison to 20% recovery in 96 h by chromatography process.The ATPE method, therefore, seems to be an interesting alternative for simultaneous partitioning and purification of phytase. The influence of system parameters; such as, phase forming salts, polymer molecular weight and system pH on the partitioning behavior of phytase was evaluated. The ATPE system consisting of combination of polyethylene glycol (PEG) 6000 and 8000(10.5%) and sodium citrate (20.5%) resulted in one-sided partitioning of phytase in bottom phase with a purification factor of 2.5. This is the first report on phytase purification using liquid-liquid extraction and the results are likely to be beneficial in the poultry feed industry. (c) 2012 Elsevier Ltd. All rights reserved.

10aAspergillus niger10aliquid-liquid extraction10aPhytase10aPolyethylene glycol10aPurification1 aBhavsar, Kavita, P.1 aKumar, Ravi1 aKhire, Jayant, Malhar uhttp://library.ncl.res.in/content/downstream-processing-extracellular-phytase-aspergillus-niger-chromatography-process-vs-002604nas a2200253 4500008004100000022001400041245018200055210006900237260005700306300001200363490000700375520164900382653001602031653002202047653001602069653001202085653002902097100002402126700001402150700001302164700001602177700002602193856013102219 2013 eng d a0175-759800aCombinatorial approach of statistical optimization and mutagenesis for improved production of acidic phytase by Aspergillus niger NCIM 563 under submerged fermentation condition0 aCombinatorial approach of statistical optimization and mutagenes a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcJAN a673-6790 v973 a

Combination of statistical optimization and mutagenesis to isolate hypersecretory strains is studied to maximize phytase production from Aspergillus niger NCIM 563 under submerged fermentation. The overall results obtained show a remarkable 5.98-fold improvement in phytase production rates when compared to that using basal medium. Optimization of culture conditions from parent strain is studied first by the Plackett-Burman technique to evaluate the effects of 11 variables for phytase production. The results showed that glucose, MgSO4, KCl, incubation period, and MnSO4 are the most significant variables affecting enzyme production. Further optimization in these variables, using a central composite design technique, resulted in 3.74-fold increase in the yield of phytase production to 254,500 U/l when compared with the activity observed with basal media (68,000 U/l) in shake flask. Our experiments show that the phytase from A. niger NCIM 563 exhibits desirable activity in simulated gastric fluid conditions with low pH and also improved thermostability when compared to commercial phytase. The improved yield demonstrates the potential applicability of phytase enzyme as a source of phytase supplement for phosphorus nutrition and environmental protection in animal feed industry. Physical and chemical mutagenesis experiments were carried out in parallel to isolate hypersecretory mutants that could possibly further enhance the enzyme production. Using optimized media conditions of the parent strain, our results show that mutant strain A. niger NCIM 1359 increased the phytase activity by another 1.6-fold to 407,200 U/l.

10aAnimal feed10aAspergillus niger10aMutagenesis10aPhytase10aStatistical optimization1 aBhavsar, Kavita, P.1 aGujar, P.1 aShah, P.1 aKumar, Ravi1 aKhire, Jayant, Malhar uhttp://library.ncl.res.in/content/combinatorial-approach-statistical-optimization-and-mutagenesis-improved-production-acidic-0