01870nas a2200217 4500008004100000022001400041245012000055210006900175260013100244300000900375490000700384520101500391653001601406653001401422653001001436653002101446653002201467100002001489700001801509856012501527 2007 eng d a0972-853800aEffect of auxin types on in vitro and ex vitro rooting and acclimatization of grapevine as influenced by substrates0 aEffect of auxin types on in vitro and ex vitro rooting and accli aDIV FRUITS & HORTICULTURAL TECHNOL, INDIAN AGRICULTURAL RESEARCH INST, NEW DELHI, 110 012, INDIAbHORTICULTURAL SOC INDIAcMAR a5-110 v643 a

Transferring micropropagated plantlets from in vitro to in vivo conditions is a critical step because plantlets have under-developed cuticle and stomata which result in wilting due to low humidity, fungal attack and bleaching in high light intensities. The resumption of terminal growth indicates successful establishment which is a change from mixotrophy to autotrophy. The survival of Vitis vinifera cvs. 2A-Clone and Red Globe plantlets rooted in vitro was 86.66 and 83.33%, respectively and the established plants showed a well-defined root system. Plantlets rooted in vivo by auxin pulse were easier to transfer and acclimatize with a survival percentage of 96.66 and 79.92 for 2A-Clone and Red Globe, respectively but the rooting was of fibrous type with few main roots and less number of root hairs. Coco-peat in combination with sand and soil was better for quick acclimatization and establishment (97.5 and 95% survival for 2A-Clone and Red Globe respectively) of the micropropagated plantlets.

10aauxin pulse10acoco-peat10agrape10amicropropagation10aplantlet survival1 aBarreto, M., S.1 aNookaraju, A. uhttp://library.ncl.res.in/content/effect-auxin-types-vitro-and-ex-vitro-rooting-and-acclimatization-grapevine-influenced02040nas a2200229 4500008004100000022001400041245011500055210006900170260012300239300001200362490000700374520115000381653001801531653000901549653001801558653002001576100001801596700002001614700002401634700002401658856012801682 2007 eng d a0042-750000aSynergistic effect of CPPU and benzyladenine on embryo rescue in six. stenospermocarpic cultivars of grapevine0 aSynergistic effect of CPPU and benzyladenine on embryo rescue in aINST REBENZUCHTUNG GEILWEILERHOF, D-76833 SIEBELDINGEN, GERMANYbBUNDESANSTALT ZUCHTUNGS FORSCHUNG KULTURPFLANZENcJAN a188-1910 v463 a

In ovulo/embryo culture technique has been used to recover hybrids from seedless grapevines. The present investigation was carried out to study the influence of pre-bloom sprays of N-(2-chloro-4-pyridyl)-N'-phenylurea (CPPU) - a synthetic cytokinin, and N-6-benzyladenine (BA) supplemented in culture media on embryo recovery in six stenospermocarpic grapevine cultivars. The results showed synergistic effect of CPPU and BA on embryo recovery. Though a CPPU spray alone increased embryo recovery in four out of six cultivars tested, the efficiency was enhanced several fold on culture of excised ovules on media supplemented with BA. The percentage of embryo recovery also depended on BA concentration and varied among six cultivars indicating a genotypic influence as well. Germination percentage of rescued embryos varied among the six cultivars and mostly corresponded with embryo recovery. Germinated embryos developed into normal plantlets. Present study demonstrates that spraying of panicles with CPPU and incorporation of BA in the ovule culture medium can enhance the embryo recovery in stenospermocarpic cultivars of grapevine.

10abenzyladenine10aCPPU10aembryo rescue10aseedless grapes1 aNookaraju, A.1 aBarreto, M., S.1 aKaribasappa, G., S.1 aAgrawal, Dinesh, C. uhttp://library.ncl.res.in/content/synergistic-effect-cppu-and-benzyladenine-embryo-rescue-six-stenospermocarpic-cultivars-002107nas a2200241 4500008004100000022001400041245013300055210006900188260012300257300001000380490000700390520118100397653001601578653001401594653001501608653001501623653001501638653002301653100001801676700002001694700002401714856012701738 2008 eng d a0042-750000aCellular polyamines influence maturation and germination of somatic embryos from pro-embryonal masses of two grapevine cultivars0 aCellular polyamines influence maturation and germination of soma aINST REBENZUCHTUNG GEILWEILERHOF, D-76833 SIEBELDINGEN, GERMANYbBUNDESANSTALT ZUCHTUNGS FORSCHUNG KULTURPFLANZENcJAN a31-340 v473 a

Somatic embryos of grapevine multiply repeatedly and often fail to mature and germinate due to factors like dormancy and embryo teratology. The present investigation was carried out to improve the maturation and germination of somatic embryos from pro-embryonal masses (PEM) of 2A-Clone and `Crimson Seedless', two seedless grapevine cultivars. Also, the aim was to study a correlation between cellular and residual polyamine (PA) levels in PEM and culture media. The efficiency of maturation and germination of embryos from PEM varied significantly between the two cultivars and depended on incubation period and type of PAs in the medium. HPLC analysis showed that higher levels of cellular putrescine in PEM had correlation with maturation and germination percentages in both cultivars. The levels of three PAs depleted in the media rapidly indicating its uptake by PEM. Of the three PAs, putrescine (PUT) was the most effective and resulted in 100.0 or 92.0 % maturation at 14 d or 30 d in 2A-Clone or `Crimson Seedless', respectively. The maximum germination of somatic embryos was recorded with PUT at 14 d or 21 d in 2A-Clone or `Crimson Seedless', respectively.

10agermination10aGrapevine10amaturation10apolyamines10a`2A-Clone'10a`Crimson Seedless'1 aNookapaju, A.1 aBarreto, M., S.1 aAgrawal, Dinesh, C. uhttp://library.ncl.res.in/content/cellular-polyamines-influence-maturation-and-germination-somatic-embryos-pro-embryonal-002838nas a2200301 4500008004100000020002200041022001400063245007100077210006900148260030100217300001200518520163500530653001602165653001402181653002202195653002102217653001902238653001002257100002002267700001802287700002102305700002402326700002402350700001902374700001902393700002302412856010102435 2008 eng d a978-90-6605-268-0 a0567-757200aVariability among vitis vinifera cultivars to in vitro propagation0 aVariability among vitis vinifera cultivars to in vitro propagati aPo Box 500, 3001 Leuven 1, BelgiumbAgr & Processed Food Prod Export Dev Author; Indian Farmers Fertilizer Cooperat Ltd; Maharashtra Agro Industries Dev Corp Ltd; Indian Council Agr Res; Natl Res Ctr Grapes; Federat Indian Chambers Commerce & Ind; Natl Hort Board; Minist Food Proc IndustriecMAY a127-1393 a

Response of grapevines to tissue culture has been reported to be genotype dependent, hence it becomes imperative to optimize culture conditions for newly developed varieties or cultivars, needing a large scale planting but availability of sufficient planting stock is a constraint. In the present study, conditions for in vitro propagation of six popular table grape cultivars have been standardized. Single node stem segments of cultivars 2A-Clone of Thompson Seedless, Red Globe, Crimson Seedless, Thompson Seedless, Flame Seedless and Italia cultured on ten different basal media showed varied percentages of bud break and morphogenetic responses. The percentage of bud break varied among the six cultivars. Direct rooting at basal ends of single node segments was observed in all the cultivars except in Italia. Woody plant medium induced the highest response and Eriksson medium the least in all the cultivars tested. Rooted nodal segments with shoots in axils could be established into whole plants on potting. Six cultivars showed different optimum concentrations of growth regulators for induction of maximum number of multiple shoots in both primary as well as secondary nodal segments. Shoot proliferation could be enhanced by several fold in the majority of cultivars on culture of initial shoot clumps to glass bottles instead of culture tubes. Six cultivars showed significant differences in optimum requirement of nutrients and growth regulators for shoot elongation, in vitro and ex vitro rooting and survival rate. Tissue culture plants of all the six cultivars could establish successfully in a greenhouse.

10abasal media10aGrapevine10agrowth regulators10amicropropagation10aTissue culture10aVitis1 aBarreto, M., S.1 aNookaraju, A.1 aJoglekar, A., M.1 aKaribasappa, G., S.1 aAgrawal, Dinesh, C.1 aAdsule, P., G.1 aSawant, I., S.1 aShikhamany, S., D. uhttp://library.ncl.res.in/content/variability-among-vitis-vinifera-cultivars-vitro-propagation-0