02150nas a2200265 4500008004100000022001400041245013600055210006900191260010200260300001000362490000800372520114400380653002401524653001101548653001501559653002501574653001701599100002401616700002501640700001601665700002601681700002801707700002201735856012701757 2014 eng d a0011-389100aCulturable bacterial phylogeny from a shallow water hydrothermal vent of espalamaca (Faial, Azores) reveals a variety of novel taxa0 aCulturable bacterial phylogeny from a shallow water hydrothermal aC V RAMAN AVENUE, SADASHIVANAGAR, P B \#8005, BANGALORE 560 080, INDIAbINDIAN ACAD SCIENCEScJAN a58-690 v1063 a

Phylogenetic diversity of the 16S rRNA gene associated with the domain bacteria was examined at the level of operational taxonomic units (OTUs) using the rarefaction analysis from a newly identified shallow water hydrothermal vent, Espalamaca in the Azorean Island Faial (Horta), North Atlantic Ocean. Diluted sea water nutrient agar amended with metals manganese, lead, iron and element sulphur, could help in retrieving highest OTUs (95) from the vent and 39 OTUs from nonvent. Molecular tools implemented on bacterial census indicated the occupancy of gamma-Proteobacteria by 55.78%, alpha-Proteobacteria by 21.05% and 12.63% of Bacteroidetes in the total population. Occurrence of novel species maximized with alpha-Proteobacteria (11/20) followed by Bacteroidetes (5/12) signified the necessity of studying these groups to strengthen the biodiversity database. Shannon index (H') and the Chao I richness estimator illustrated a strong bacterial community in the venting area. The current study confers many bacterial genera which were not reported earlier in any of the shallow water vents and adds 33 new taxa to the database.

10aBacterial phylogeny10ametals10anovel taxa10ararefaction analysis10ashallow vent1 aRajasabapathy, Raju1 aMohandass, Chellandi1 aColaco, Ana1 aDastager, Syed, Gulam1 aSantos, Ricardo, Serrao1 aMeena, Ram, Murti uhttp://library.ncl.res.in/content/culturable-bacterial-phylogeny-shallow-water-hydrothermal-vent-espalamaca-faial-azores-002667nas a2200277 4500008004100000022001400041245008000055210006900135260007400204300001200278490000800290520174300298653001502041653002402056653003302080653001302113100002402126700002502150700002602175700001402201700002002215700001702235700001602252700001602268856010502284 2014 eng d a0003-607200aRoseovarius azorensis sp nov., isolated from seawater at espalamaca, azores0 aRoseovarius azorensis sp nov isolated from seawater at espalamac aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcMAR a571-5780 v1053 a

A Gram-negative, motile, non-spore forming, rod shaped aerobic bacterium, designated strain SSW084(T), was isolated from a surface seawater sample collected at Espalamaca (38A degrees 33'N; 28A degrees 39'W), Azores. Growth was found to occur from 15 to 40 A degrees C (optimum 30 A degrees C), at pH 7.0-9.0 (optimum pH 7.0) and with 25-100 % seawater or 0.5-7.0 % NaCl in the presence of Mg2+ and Ca2+; no growth was found with NaCl alone. Colonies on seawater nutrient agar were observed to be punctiform, white, convex, circular, smooth, and translucent. Strain SSW084(T) did not grow on Zobell marine agar and tryptic soy agar even when seawater supplemented. The major respiratory quinone was found to be Q-10 and the G + C content was determined to be 61.9 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain SSW084(T) belongs to the genus Roseovarius and that its closest neighbours are Roseovarius tolerans EL-172(T), Roseovarius mucosus DFL-24(T) and Roseovarius lutimaris 112(T) with 95.7, 95.4 and 95.3 % sequence similarity respectively. The remaining species of Roseovarius showed < 95 % similarity. The polar lipids of strain SSW084(T) were determined to be phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified lipid and one unidentified aminolipid. The major fatty acids identified were identified as C-18:1 omega 7c (52.5 %) and C-16:0 (13.8 %). On the basis of phenotypic, molecular and chemotaxonomic characteristics, strain SSW084(T) is considered to represent a novel species of the genus Roseovarius, for which Roseovarius azorensis sp. nov is proposed. The type strain is SSW084(T) (=KCTC 32421(T) = MTCC 11812(T)).

10aEspalamaca10aPolyphasic taxonomy10aRoseovarius azorensis sp nov10aSeawater1 aRajasabapathy, Raju1 aMohandass, Chellandi1 aDastager, Syed, Gulam1 aLiu, Qing1 aKhieu, Thi-Nhan1 aSon, Chu, Ky1 aLi, Wen-Jun1 aColaco, Ana uhttp://library.ncl.res.in/content/roseovarius-azorensis-sp-nov-isolated-seawater-espalamaca-azores-002578nas a2200265 4500008004100000022001400041245014800055210006900203260007400272300001400346490000800360520159000368653001101958653003701969653002202006653002402028653001302052100002402065700002502089700002602114700001402140700001602154700001602170856012602186 2015 eng d a0003-607200aCitreicella manganoxidans sp nov., a novel manganese oxidizing bacterium isolated from a shallow water hydrothermal vent in Espalamaca (Azores)0 aCitreicella manganoxidans sp nov a novel manganese oxidizing bac aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcDEC a1433-14390 v1083 a

A Gram-stain negative, non-motile, non-spore forming, aerobic and rod or narrow lemon-shaped bacterial strain, VSW210(T), was isolated from surface seawater in a shallow water hydrothermal vent region in Espalamaca (Azores). Strain VSW210(T) was found to grow optimally at 30 A degrees C, at pH 7 and in the presence of 2-6 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain VSW210(T) clusters with the type strain Citreicella marina CK-I3-6(T) (sequence similarity value of 99.6 %), but DNA-DNA hybridization showed DNA-DNA relatedness between the strain VSW210(T) and C. marina CK-I3-6(T) to be 55.8 +/- A 3.2 %. The DNA G+C content of strain VSW210(T) was determined to be 67.4 mol%. The cellular fatty acid profiles of strain VSW210(T) was found to contain C-18:1 omega 7c (80.1 %) and C-16:0 (9.2 %). The major polar lipids in strain VSW210(T) were identified as phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified phospholipid. Strain VSW210(T) was found to be able to oxidize soluble Mn(II) to insoluble MnO2, which was confirmed with LBB staining. Differential phenotypic properties and genetic uniqueness revealed that this strain VSW210(T) is distinguishable from other species of the genus Citreicella. On the basis of the data presented, strain VSW210(T) is considered to represent a novel species of the genus Citreicella, for which the name Citreicella manganoxidans sp. nov. is proposed. The type strain is VSW210(T) (=KCTC 32497(T) = MCC 2286(T)).

10aAzores10aCitreicella manganoxidans sp nov10aHydrothermal vent10aPolyphasic taxonomy10aSeawater1 aRajasabapathy, Raju1 aMohandass, Chellandi1 aDastager, Syed, Gulam1 aLiu, Qing1 aLi, Wen-Jun1 aColaco, Ana uhttp://library.ncl.res.in/content/citreicella-manganoxidans-sp-nov-novel-manganese-oxidizing-bacterium-isolated-shallow-002521nas a2200301 4500008004100000022001400041245012300055210006900178260000800247300001200255490000800267520152700275653001501802653002801817653002801845653002401873653001301897100002401910700002501934700002001959700002601979700001402005700002002019700001702039700001602056700001602072856013102088 2015 eng d a0003-607200aNioella nitratireducens gen. nov., sp nov., a novel member of the family Rhodobacteraceae isolated from Azorean Island0 aNioella nitratireducens gen nov sp nov a novel member of the fam cFEB a589-5950 v1073 a

A novel Gram-negative, non-spore forming, rod-shaped aerobic bacterium, designated SSW136(T), was isolated from a surface seawater sample collected at Espalamaca (in Faial Island), Azores. Growth was found to occur from 10 to 37 A degrees C, pH 6.0-8.0, and with 2-11 % of NaCl. 16S rRNA gene sequence indicated that the strain SSW136(T) belongs to the family Rhodobacteraceae. Strain SSW136(T) exhibited 96.3, 95.9, 95.7 and 95.5 sequence similarity to the type strains Oceanicola litoreus M-M22(T), Roseovarius aestuarii SMK-122(T), Marivita geojedonensis DPG-138(T), and Pseudoruegeria aquimaris SW-255(T) respectively. Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain SSW136(T) was affiliated to the family Rhodobacteraceae and formed a separate branch. The G+C content was 63.5 mol%. The major respiratory quinone was found to be Q-10. The polar lipids of strain SSW136(T) consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and three unidentified phospholipids. The major fatty acids were C-18:1 omega 7c (46.5 %), Cyclo-C-19:0 omega 8c (16.0 %) and C-16:0 (12.8 %). On the basis of the morphological, genotypic, chemotaxonomic characteristics and low DNA-DNA relatedness, strain SSW136(T) is proposed to represent a novel genus and novel species, Nioella nitratireducens gen. nov., sp. nov., in the family Rhodobacteraceae. The type strain is SSW136(T) (=KCTC 32417(T) = NCIM 5499(T)).

10aEspalamaca10aNioella nitratireducens10aNovel genus and species10aPolyphasic taxonomy10aSeawater1 aRajasabapathy, Raju1 aMohandass, Chellandi1 aYoon, Jung-Hoon1 aDastager, Syed, Gulam1 aLiu, Qing1 aKhieu, Thi-Nhan1 aSon, Chu, Ky1 aLi, Wen-Jun1 aColaco, Ana uhttp://library.ncl.res.in/content/nioella-nitratireducens-gen-nov-sp-nov-novel-member-family-rhodobacteraceae-isolated-azorean02245nas a2200241 4500008004100000022001400041245008500055210006900140260011800209300001200327490000700339520136700346100002401713700002501737700002001762700002601782700001401808700002001822700001701842700001601859700001601875856011201891 2015 eng d a1466-502600aVitellibacter nionensis sp nov., isolated from a shallow water hydrothermal vent0 aVitellibacter nionensis sp nov isolated from a shallow water hyd aMARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLANDbSOC GENERAL MICROBIOLOGYcFEB a692-6970 v653 a

A novel, Gram-stain-negative, non-motile, rod-shaped yellow bacterium, designated VBW088(T) was isolated from a shallow water hydrothermal vent in Espalamaca in the Azores. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain VBW088(T) clustered with three type strains of species of the genus Vitellibacter and exhibited a sequence similarity of 97.3% with Vitellibacter soesokkakensis RSSK-12(T). However, strain VBW088(T) and V. soesokkakensis RSSK-12(T) exhibited low DNA-DNA relatedness (12.7 +/- 3.5 %). Strain VBW088(T) was positive for catalase and oxidase. Growth occurred at 10-37 degrees C, with the optimum at 80 degrees C, and at pH 6.0-8.0 (optimum pH 6.0) and in up to 5% (w/v) NaCl with optimum growth at 1-2% (w/v) NaCl. The major fatty acids (>10%) were iso-C-15:0 (33.5%) and iso-C-17:0 3-OH (32.0%). The polar lipids detected in strain VBW088(T) consisted of phosphatidylethanolamine, one unidentified aminolipid and three unidentified phospholipids. The DNA G+C content of strain VBW088(T) was 36.7 mol%. On the basis of phylogenetic inference, DNA-DNA relatedness, chemotaxonomic analysis and physiological data, the isolate represents a novel species of the genus Vitellibacter, for which the name Vitellibacter nionensis sp. nov. is proposed, with the type strain as VBW088(T). (=KCTC 32420(T)=MCC 2354(T)).

1 aRajasabapathy, Raju1 aMohandass, Chellandi1 aYoon, Jung-Hoon1 aDastager, Syed, Gulam1 aLiu, Qing1 aKhieu, Thi-Nhan1 aSon, Chu, Ky1 aLi, Wen-Jun1 aColaco, Ana uhttp://library.ncl.res.in/content/vitellibacter-nionensis-sp-nov-isolated-shallow-water-hydrothermal-vent-002284nas a2200253 4500008004100000022001400041245013700055210006900192260007900261300001200340490000800352520132400360653001701684653002301701653003001724653002401754653001401778100002001792700002501812700002601837700002401863700002101887856012201908 2016 eng d a0273-228900aAntioxidative metabolites synthesized by marine pigmented vibrio sp and its protection on oxidative deterioration of membrane lipids0 aAntioxidative metabolites synthesized by marine pigmented vibrio a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcAPR a155-1670 v1793 a

Bacterial strain Vibrio sp. (PIGB 184) isolated from water samples of the Arabian Sea and identified through 16S rRNA demonstrated the production of pigmentary antioxidants with higher ABTS activities 90.9 +/- 0.42 % in comparison with the standard commercial pigmented antioxidant, quercetin 88.8 +/- 1.4 %. Antioxidative metabolites of this strain substantially inhibit the lipid peroxidation (LPO) reactions tested in sheep liver and brain. The antioxidant compounds produced by the Vibrio sp. (PIGB 184), analysed by GC-MS, reveals that it is composed mostly of phenol, 2,4-bis(1,1-dimethylethyl) and pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro-3-(2-methylpropyl). The interrelationship assessed between LPO and the phenolic compounds showed significant correlation with anti-LPO properties (R (2) = 0.9698 to 0.9861). These compounds are responsible for obstruction of harmful radical associated biochemical reactions in biological systems. Pigmented metabolites also tested for attributive biological properties against pathogenic bacteria showed prominent inhibition towards Gram-positive organisms (31.25 to 62.5 mu g ml(-1)). From this study, it may be suggested that the marine bacterium PIGB 184 could be used as a potential bio-resource for antioxidants and needs to be worked out for mass production.

10aAntioxidants10aLipid peroxidation10aMarine pigmented bacteria10aPathogen inhibition10aPhenolics1 aPawar, Ravindra1 aMohandass, Chellandi1 aDastager, Syed, Gulam1 aKolekar, Yogesh, M.1 aMalwankar, Rahul uhttp://library.ncl.res.in/content/antioxidative-metabolites-synthesized-marine-pigmented-vibrio-sp-and-its-protection02412nas a2200289 4500008004100000022001400041245010300055210006900158260000800227300001100235490000800246520145800254653001401712653002701726653002401753653001501777653001101792100002401803700002301827700002901850700002501879700002201904700002301926700001901949700002801968856012601996 2020 eng d a0882-401000aAntimicrobial profiling of coral reef and sponge associated bacteria from southeast coast of India0 aAntimicrobial profiling of coral reef and sponge associated bact cAPR a1039720 v1413 a

Culturable bacteria associated with marine sponges and coral mucus (collected from Gulf of Mannar and Palk Bay) were screened for their prospective antimicrobial compounds against 9 bacterial pathogens (Bacillus megaterium, B. cereus, Salmonella typhimurium, Staphylococcus aureus, Proteus vulgaris, Klebsillla pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumannii) and a fungal pathogen (Candida albicans). Of the 263 bacterial isolates obtained during this study, 52 isolates displayed antimicrobial activity against one or more pathogens. 16S rRNA gene sequencing revealed that these 52 strains affiliated to 14 genera from three phyla Proteobacteria, Firmicutes and Actinobacteria. Sponge associated bacterial strains F-04, 1-23, 1-33 and G-03 inhibited the growth of all the bacterial pathogens tested in this study and significantly the former 2 strains inhibited the growth of fungal pathogen also. Majority of the potential strains (88.4% out of 52 strains) inhibited the growth of Bacillus cereus. Interestingly, an actinomycete strain F-04 (isolated from sponge Orina sagittaria) inhibited the growth of methicillin resistant Staphylococcus aureus. In total, 10 volatile organic compounds were determined from the ethyl acetate and hexane extract of the strain F-04 using GC-MS. Overall, marine bacteria isolated during this study demonstrate the potential for the development of broad spectrum antibiotics.

10aAnti-MRSA10aAntimicrobial activity10aBacterial diversity10aCoral reef10aSponge1 aRajasabapathy, Raju1 aGhadi, Sanjeev, C.1 aManikandan, Balakrishnan1 aMohandass, Chellandi1 aSurendran, Akhila1 aDastager, Syed, G.1 aMeena, Ram, M.1 aJames, Rathinam, Arthur uhttp://library.ncl.res.in/content/antimicrobial-profiling-coral-reef-and-sponge-associated-bacteria-southeast-coast-india