01443nas a2200241   4500008004100000022001400041245013100055210006900186260007300255300001200328490000700340520057600347653001500923653001300938653001100951653001100962653001700973100002100990700002001011700002001031700002301051856012701074       2008                        eng d  a0141-813000aEnvironment friendly crosslinked chitosan as a matrix for selective adsorption and purification of lipase of aspergillus niger0 aEnvironment friendly crosslinked chitosan as a matrix for select  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcDEC  a422-4250 v433 a<p>Chitosan and its derivatives have been used as affinity matrices for purification of lipase from Aspergillus niger NCIM 1207. Trimellitic anhydride (TMA)-crosslinked deacetylated chitin adsorbed lipase selectively, yielding approximately 5-fold purification of the crude lipase with 70% yield. Further 9-fold purification occurred on eluting through Sephacryl-100. These results suggest that chitosan derivatives can be used as inexpensive biopolymer matrices for the purification of lipases for industrial applications. (C) 2008 Elsevier B.V. All rights reserved.</p>10aAdsorption10aChitosan10aLipase10aMatrix10aPurification1 aTrimukhe, K., D.1 aMahadik, N., D.1 aGokhale, D., V.1 aVarma, Anjanikumar  uhttp://library.ncl.res.in/content/environment-friendly-crosslinked-chitosan-matrix-selective-adsorption-and-purification-002052nas a2200193   4500008004100000245010100041210006900142260000800211300001400219490000700233520136200240100002001602700001801622700002001640700002101660700002301681700002801704856012601732       2016                        eng d00aEffect of mixed acid catalysis on pretreatment and enzymatic digestibility of sugar cane bagasse0 aEffect of mixed acid catalysis on pretreatment and enzymatic dig  cSEP  a7310-73180 v303 aAqueous pretreatment using homogeneous acid catalyst is considered as a low-cost technology in the production of lignocellulosic bioethanol. To establish the synergism of mixed acids, pilot-level aqueous pretreatments of bagasse covering a wide range of combined severity (CS) were carried out. To investigate the effect of application of mixture of acids on xylose hydrolysis as well as glucose hydrolysis via pretreatment and enzymatic hydrolysis, the following three combinations of acids were explored: (1) oxalic acid + sulfuric acid (organic + mineral acid), (2) phosphoric + sulfuric acid (mineral acids), and (3) ferric chloride + sulfuric acid (Lewis acid with a mineral acid). Of the pretreatments evaluated, the synergism was most pronounced for the combination of sulfuric and phosphoric acid, which resulted in more than 90% conversion of hemicellulose to xylose and 70% conversion of cellulose to glucose through enzymatic hydrolysis. Fourier transform infrared (FTIR) studies of pretreated samples showed higher syringyl/guaiacyl (S/G) ratio for sulfuric and phosphoric acid combination pretreatment, leading to higher enzymatic conversion. FTIR and dynamic light scattering (DLS) experiments conducted on pretreated sugar cane bagasse provided useful correlation with regard to the pretreatment type, particle size, and enzymatic hydrolysis.1 aPal, Siddhartha1 aJoy, Shereena1 aKumbhar, Pramod1 aTrimukhe, K., D.1 aVarma, Anjanikumar1 aPadmanabhan, Sasisanker  uhttp://library.ncl.res.in/content/effect-mixed-acid-catalysis-pretreatment-and-enzymatic-digestibility-sugar-cane-bagasse