02259nas a2200193   4500008004100000022001400041245015000055210006900205260007800274300001200352490000700364520144100371100001801812700002001830700002401850700003001874700003301904856012801937       2011                        eng d  a1462-031600aRecurrent somatic embryogenesis and plant regeneration in Angelica glauca Edgew., a critically endangered medicinal plant of the Western Himalaya0 aRecurrent somatic embryogenesis and plant regeneration in Angeli  aINVICTA PRESS, ASHFORD TN24 8HH, KENT, ENGLANDbHEADLEY BROTHERS LTDcSEP  a493-4980 v863 a<p>Secondary somatic embryogenesis and plant regeneration from seedling explants of Angelica glauca, an endangered medicinal plant of the Himalaya, is reported for the first time. Callus was obtained from all the explants tested in the present study (i.e., epicotyls, hypocotyls, and cotyledonary nodes). The highest frequency of callus formation (95.8%) was observed using epicotyl explants on 4.0 mu M 2,4-dichlorophenoxyacetic acid (2,4-D), whereas 70.8% of hypocotyl explants, and 58.3% of cotyledonary nodes produced callus. One-hundred percent embryogenic callus was induced from epicotyl explants in 2.0 mu M 6-benzyladenine (BA) and 2.0 mu M alpha-naphthaleneacetic acid (NAA), together with the maximum number of somatic embryos (34.2 embryos per explant). Cotyledonary nodes did not produce somatic embryos. Histological studies confirmed the induction of somatic embryogenesis. Somatic embryos germinated into plantlets upon transfer to half-strength Murashige and Skoog (MS) medium without added plant growth regulators. We observed 85% survival of these plantlets under field conditions. The development of secondary embryos was also observed when primary embryos were sub-cultured on full-strength MS medium containing 2.0 mu M NAA plus 2.0 mu M BA. This system of recurrent somatic embryogenesis provides a route for gene transfer and also for the large-scale production of this critically endangered medicinal plant.</p>1 aPandey, Meena1 aDhar, Uppeandra1 aSamant, Sher, Singh1 aShirgurkar, Mrudul, Vijay1 aThengane, Shubhada, Ratnakar  uhttp://library.ncl.res.in/content/recurrent-somatic-embryogenesis-and-plant-regeneration-angelica-glauca-edgew-critically-002660nas a2200241   4500008004100000022001400041245022700055210006900282260000900351520169200360653002002052653001002072653002202082653002602104653001902130653002602149100001802175700002602193700002902219700002402248700002002272856012602292       2020                        eng d  a0721-759500aCombinatorial approach through in vitro regeneration and phytochemical profiling of ceropegia media (huber) ans.: a potential way forward in the conservation of an endangered medicinal plant from the western ghats in India0 aCombinatorial approach through in vitro regeneration and phytoch  cJUN 3 a<p>Ceropegia mediais an endemic and endangered plant as its propagation through seeds is unreliable due to low germination, slow growth and seedling decay under natural conditions. Also, tubers of this plant are edible serving as carbohydrate source with medicinal values leading to severe population decline in the natural habitat. To provide a sustainable solution, an efficient in vitro propagation protocol along with phytochemical profiling was developed forC. media. Callus cultures were induced from seedling and wild leaf tissues using the most effective Murashige and Skoog's (MS) medium with 2,4-dichlorophenoxyacetic acid (2,4-D; 2 mu M) and sucrose (3%). Somatic embryos were acquired on MS medium with 1 mu M 6-Benzylaminopurine (BAP) and 1 mu M 2,4-D. Conversion into plantlets was attained only from tissue culture-derived seedling leaf (TCDSL) explant. Further, in vitro tuberization was achieved from TCDSL callus with BAP and Naphthalene acetic acid (NAA). AgNO(3)as an elicitor had a positive effect on both fresh and dry weights of callus. Successful acclimatization (58%) was attained after two months resulting in normal phenotype in pots. Further, metabolite profiles of ten different tissues from wild and in vitro plants were compared. Total 82 compounds comprising alkaloids, fatty acids, fatty acid ester, steroids, terpenes and hydrocarbons were identified. Overall, results suggested enhanced production of selected metabolites with in vitro propagation and AgNO3, alleviating the problem of unavailability of planting materials. Thus, the current study might offer potential ways for the conservation of such RED enlisted species asC. media. [GRAHICS]</p>
10aCeropegia media10aGC-MS10aMicrotuberization10aSecondary metabolites10aSilver nitrate10asomatic embryogenesis1 aPandey, Meena1 aDholakia, Bhushan, B.1 aJayaramaiah, Ramesha, H.1 aPunekar, Sachin, A.1 aGiri, Ashok, P.  uhttp://library.ncl.res.in/content/combinatorial-approach-through-vitro-regeneration-and-phytochemical-profiling-ceropegia