02071nas a2200265   4500008004100000022001400041245011400055210006900169260011700238300001000355490000600365520106900371653002801440653001501468653003701483653001701520653001401537653001101551100003101562700002401593700002001617700002201637700002301659856012301682       2007                        eng d  a0973-626300aImmobilization of invertase on functionalized mesoporous silica and properties of the immobilized biocatalyst0 aImmobilization of invertase on functionalized mesoporous silica   aSECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIAbRESEARCH JOURNAL BIOTECHNOLOGYcFEB  a12-170 v23 a<p>Invertase, which catalyses the hydrolysis of sucrose, is one of the simplest commercial carbohydrases and is used for the production of invert syrup (mixture of glucose and fructose). Fructose is the sweetest natural sugar having beneficial medicinal effects and is emerging as an alternative sweetener to sucrose in the pharmaceutical industry. beta fructofuranosidase (E C 3.2.1.26) purified from Kluyveromyces marxianus NCYC 2675, a thermotolerant yeast, was immobilized on functionalized mesoporous silica SBA- 15. Attempts were made to immobilize invertase on functionalized silicalite and cross linked with glutaraldehyde so as to obtain a highly active and stable immobilized enzyme preparation suitable for commercial exploitation. Cross linked enzyme silica complex as well as enzyme silica complex showed very good temperature and pH stability. Extrudates of the mesoporous silica also showed similar results. Bound enzyme showed excellent stability to repeated use and retained 90% of its initial activity after ten cycles of reuse in batch mode.</p>10abeta fructofuranosidase10aextrudates10afunctionalized mesoporous silica10aInvert syrup10ainvertase10aSBA-151 aPrabhune, Asmita, Ashutosh1 aSulabha, Karandikar1 aArchana, Pundle1 aShilpa, Deshpande1 aSanjeevani, Pardhy  uhttp://library.ncl.res.in/content/immobilization-invertase-functionalized-mesoporous-silica-and-properties-immobilized02007nas a2200229   4500008004100000022001400041245016100055210006900216260011700285300001000402490000600412520109600418653001501514653001901529653001901548653001401567653001301581653001601594100002401610700002101634856012201655       2012                        eng d  a0973-626300aGellan Gum a novel polysaccharide matrix for immobilization of thermo-tolerant yeast cells with invertase activity: factorial design and rheological studies0 aGellan Gum a novel polysaccharide matrix for immobilization of t  aSECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIAbRESEARCH JOURNAL BIOTECHNOLOGYcNOV  a81-870 v73 a<p>The microbial extracellular polysaccharide gellan gum has been explored for its application in whole cell immobilization. Preliminary evaluation of the physicochemical properties of gellan gum in the presence of monovalent cation tetramethyl ammonium chloride (TMACl) was carried out. Gellan gum immobilized cells demonstrated better rheological properties than gellan gum. Attempts have been made to reveal the evaluation of gellan gum as a matrix to immobilize a thermotolerant yeast Kluyveromyces marxianus NCYC 2675. A 3(2) factorial design was used to study the simultaneous effect of two variables. The effect of the polymer concentration and TMACl concentration on various dependent variables like gelling temperature, mean particle size and enzyme activity of the yeast cells was studied. A statistical model with a significant interaction term was obtained to predict the results. Further, optimized immobilized system was evaluated with significant storage stability at 10 C and enhanced reusability with maximum conversion of &gt; 90 % when crosslinked with glutaraldehyde.</p>10aGellan gum10aGlutaraldehyde10aImmobilization10ainvertase10aRheology10ayeast cells1 aSulabha, Karandikar1 aAsmita, Prabhune  uhttp://library.ncl.res.in/content/gellan-gum-novel-polysaccharide-matrix-immobilization-thermo-tolerant-yeast-cells-0