02100nas a2200253   4500008004100000022001400041245015000055210006900205260009900274300001400373490000800387520111000395653001901505653003601524653004101560653001201601653001101613100001801624700002301642700002101665700002001686700001401706856012601720       2011                        eng d  a0960-852400aMicrobial production of xylitol from D-xylose and sugarcane bagasse hemicellulose using newly isolated thermotolerant yeast Debaryomyces hansenii0 aMicrobial production of xylitol from Dxylose and sugarcane bagas  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcFEB  a3304-33080 v1023 a<p>A thermotolerant yeast capable of fermenting xylose to xylitol at 40 C was isolated and identified as a strain of Debaryomyces hansenii by ITS sequencing. This paper reports the production of xylitol from D-xylose and sugarcane bagasse hemicellulose by free and Ca-alginate immobilized cells of D. hansenii. The efficiency of free and immobilized cells were compared for xylitol production from D-xylose and hemicellulose in batch culture at 40 degrees C. The maximum xylitol produced by free cells was 68.6 g/L from 100 g/L of xylose, with a yield of 0.76 g/g and volumetric productivity 0.44 g/L/h. The yield of xylitol and volumetric productivity were 0.69 g/g and 0.28 g/L/h respectively from hemicellulosic hydrolysate of sugarcane bagasse after detoxification with activated charcoal and ion exchange resins. The Ca-alginate immobilized D. hansenii cells produced 73.8 g of xylitol from 100 g/L of xylose with a yield of 0.82 g/g and volumetric productivity of 0.46 g/L/h and were reused for five batches with steady bioconversion rates and yields. (C) 2010 Elsevier Ltd. All rights reserved.</p>10aImmobilization10aSugarcane bagasse hemicellulose10aThermotolerant Debaryomyces hansenii10axylitol10aXylose1 aPrakash, Gyan1 aVarma, Anjanikumar1 aPrabhune, Asmita1 aShouche, Yogesh1 aRao, Mala  uhttp://library.ncl.res.in/content/microbial-production-xylitol-d-xylose-and-sugarcane-bagasse-hemicellulose-using-newly-002604nas a2200265   4500008004100000245008000041210006900121260001200190300001200202490000700214520178100221653002302002653000902025653002402034653001702058100002102075700001802096700001902114700002302133700001902156700001902175700002002194700001902214856010502233       2017                        eng d00aMultilocus sequence typing of Ochrobactrum spp. isolated from gastric niche0 aMultilocus sequence typing of Ochrobactrum spp isolated from gas  cMAR-APR  a201-2100 v103 aThe human stomach is colonized by diverse bacterial species. The presence of non-Helicobacter pylori bacteria in urease-positive biopsies of individuals has been reported. Bacteria belonging to the Ochrobactrum genus have been documented in the human gastric niche. The co-occurrence of Ochrobactrum spp. with H. pylori was previously reported in an antral biopsy of a non-ulcer dyspeptic (NUD) subject from Northern India. There is no information on the genetic diversity of Ochrobactrum spp. isolated from the gastric niche in the stomach. We aimed to study the species distribution and diversity of Ochrobactrum spp. with and without H. pylori in urease-positive biopsies across three different geographical regions in India. Sixty-two Ochrobactrum isolates recovered from patients with an upper gastric disorder (n=218) were subjected to molecular identification and multilocus sequence typing. H. pylori DNA was found in the majority of biopsies, which had a variable degree of Ochrobactrum spp present. Interestingly, some of the urease-positive biopsies only had Ochrobactrum without any H. pylori DNA. Based on phylogenetic analysis, the Ochrobactrum isolates were distributed into the O. intermedium, O. anthropi and O. oryzae groups. This indicates there are multiple species in the gastric niche irrespective of the presence or absence of H. pylori. Antibiotyping based on colistin and polymyxin B could differentiate between O. intermedium and O. anthropi without revealing the resistance-driven diversity. Considering the prevalence of multiple Ochrobactrum spp. in the human gastric niche, it is important to evaluate the commensal and/or pathogenic nature of non-H. pylori bacteria with respect to their geographical distribution, lifestyle and nutrition needs.10aHelicobater Pyroli10aMLST10aNon-Ulcer Dyspepsia10aOchrobactrum1 aKulkarni, Girish1 aGohil, Kushal1 aMisra, Vatsala1 aKakrani, Arjun, L.1 aMisra, Sri, P.1 aPatole, Milind1 aShouche, Yogesh1 aDharne, Mahesh  uhttp://library.ncl.res.in/content/multilocus-sequence-typing-ochrobactrum-spp-isolated-gastric-niche