02538nas a2200229   4500008004100000245014400041210006900185260000800254300001800262490000700280520158900287653013501876100001802011700002402029700002802053700001802081700002302099700002302122700002102145700001702166856012502183       2012                        eng d00aNew chemodosimetric reagents as ratiometric probes for cysteine and homocysteine and possible detection in living cells and in blood plasma0 aNew chemodosimetric reagents as ratiometric probes for cysteine   cNOV  a15382–153930 v183 a<p>In this work, we have rationally designed and synthesized two new reagents (L1 and L2), each bearing a pendant aldehyde functionality. This aldehyde group can take part in cyclization reactions with β- or γ-amino thiols to yield the corresponding thiazolidine and thiazinane derivatives, respectively. The intramolecular charge-transfer (ICT) bands of these thiazolidine and thiazinane derivatives are distinctly different from those of the molecular probes (L1 and L2). Such changes could serve as a potential platform for using L1 and L2 as new colorimetric/fluorogenic as well as ratiometric sensors for cysteine (Cys) and homocysteine (Hcy) under physiological conditions. Both reagents proved to be specific towards Cys and Hcy even in the presence of various amino acids, glucose, and DNA. Importantly, these two chemodosimetric reagents could be used for the quantitative detection of Cys present in blood plasma by using a pre-column HPLC technique. Such examples are not common in contemporary literature. MTT assay studies have revealed that these probes have low cytotoxicity. Confocal laser scanning micrographs of cells demonstrated that these probes could penetrate cell membranes and could be used to detect intracellular Cys/Hcy present within living cells. Thus, the results presented in this article not only demonstrate the efficiency and specificity of two ratiometric chemodosimeter molecules for the quantitative detection of Cys and Hcy, but also provide a strategy for developing reagents for analysis of these vital amino acids in biological samples.</p>10acharge transfer; chemodosimetric detection; cysteine/ homocysteine sensors; fluorescent probes; imaging agents; ratiometric probes1 aDas, Priyadip1 aMandal, Amal, Kumar1 aChandar, Nellore, Bhanu1 aBaidya, Mithu1 aBhatt, Harshad, B.1 aGanguly, Bishwajit1 aGhosh, Sudip, K.1 aDas, Amitava  uhttp://library.ncl.res.in/content/new-chemodosimetric-reagents-ratiometric-probes-cysteine-and-homocysteine-and-possible01770nas a2200217   4500008004100000022001400041245013700055210006900192260011000261300001600371490000700387520090800394100001701302700002301319700001801342700001801360700001501378700002101393700001701414856012101431       2013                        eng d  a1477-922600aNew chemodosimetric probe for the specific detection of Hg2+ in physiological condition and its utilisation for cell imaging studies0 aNew chemodosimetric probe for the specific detection of Hg2 in p  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcJUL  a15097-151050 v423 a<p>A dithiane derivative of BODIPY is synthesized. This new reagent could be used for the specific and instantaneous detection of Hg2+ in physiological condition. This dithiane reagent reacts specifically with Hg2+ to regenerate the parent BODIPY-aldehyde with consequential change in visually detectable optical responses and this provides the possibility of using this reagent as a colorimetric probe or as a fluorescent biomarker/imaging reagent. Further, non-covalent interactions could be utilized for formation of an inclusion complex with biologically benign beta-cyclodextrin for enhancing its solubility in aqueous environment and this included adduct could be used as a fluorescent marker and imaging reagent for Hg2+. Uptake of Hg2+ ions in live HeLa cells, exposed to a solution having Hg2+ ion concentration as low as 2 ppb, could also be detected by confocal laser microscopic studies.</p>1 aSaha, Sukdeb1 aAgarwalla, Hridesh1 aGupta, Hariom1 aBaidya, Mithu1 aSuresh, E.1 aGhosh, Sudip, K.1 aDas, Amitava  uhttp://library.ncl.res.in/content/new-chemodosimetric-probe-specific-detection-hg2-physiological-condition-and-its-0