02973nas a2200181   4500008004100000245012600041210006900167260000800236300001000244490000800254520230300262100002002565700001902585700002002604700002002624700002502644856012202669       2018                        eng d00aProteomic study of endothelial dysfunction induced by Ages and its possible role in diabetic cardiovascular complications0 aProteomic study of endothelial dysfunction induced by Ages and i  cSEP  a69-790 v1873 a<p>Endothelial dysfunction is one of the primary steps in the development of diabetes associated cardiovascular diseases. Hyperglycemic condition in diabetes promotes accumulation of advanced glycation end products (AGEs) in the plasma, that interact with the receptor for AGEs (RAGE) present on the endothelial cells and negatively affect their function. Using Human umbilical vascular endothelial cells (HUVECs) in culture, the effect of glycated human serum albumin on global proteomic changes was studied by SWATH-MS, a label free quantitative proteomic approach. Out of the 1860 proteins identified, 161 showed higher abundance while 123 showed lesser abundance in cells treated with glycated HSA. Bioinformatic analysis revealed that the differentially regulated proteins were involved in various processes such as apoptosis, oxidative stress etc. that are associated with endothelial dysfunction. Furthermore, the iRegulon analysis and immunofuorescence studies indicated that several of the differentially regulated proteins were transcriptionally regulated by NF-kappa B, that is downstream to AGE-RAGE axis. Some of the important differentially regulated proteins include ICAM1, vWF, PAI-1 that affect important endothelial functions like cell adhesion and blood coagulation. qPCR analysis showed an increase in expression of the AGE receptor RAGE along with other genes involved in endothelial function. AGE treatment to HUVEC cells led to increased oxidative stress and apoptosis. This is the first proteomics study that provides insight into proteomic changes downstream to AGE-RAGE axis leading to endothelial dysfunction and predisposing to cardiovascular complications. Significance: Cardiovascular disease (CVD) is a major pathological outcome in diabetic patients and it is important to address ways that target its development before the onset. Elevated plasma AGEs in diabetes can affect endothelial function and can continue to show their effects even after blood glucose levels are back to normal. Since endothelial dysfunction acts as one of the initiating factors for the development of CVD, understanding how AGEs affect the endothelial cell proteome to cause dysfunction will provide insight into the mechanisms involved and aid designing new therapeutic approaches.</p>
1 aBanarjee, Reema1 aSharma, Akshay1 aBai, Shakuntala1 aDeshmukh, Arati1 aKulkarni, Mahesh, J.  uhttp://library.ncl.res.in/content/proteomic-study-endothelial-dysfunction-induced-ages-and-its-possible-role-diabetic01873nas a2200337   4500008004100000022001400041245012900055210006900184260001100253300001100264490000700275520074300282653002601025653002001051653003101071653004201102653001501144100001701159700001901176700001901195700002101214700002001235700001701255700002701272700002001299700002401319700001801343700001701361700002701378856013001405       2021                        eng d  a2234-943X00aPlasma-derived extracellular vesicles reveal galectin-3 binding protein as potential biomarker for early detection of glioma0 aPlasmaderived extracellular vesicles reveal galectin3 binding pr  cNOV 26  a7787540 v113 a{Gliomas are the most common type of the malignant brain tumor, which arise from glial cells. They make up about 40% of all primary brain tumors and around 70% of all primary malignant brain tumors. They can occur anywhere in the central nervous system (CNS) and have a poor prognosis. The average survival of glioma patients is approximately 6-15 months with poor aspects of life. In this edge, identification of proteins secreted by cancer cells is of special interest because it may provide a better understanding of tumor progression and provide early diagnosis of the diseases. Extracellular vesicles (EVs) were isolated from pooled plasma of healthy controls (n=03) and patients with different grades of glioma (Grade I or II or III10ablood-based biomarker10aearly detection10agalectin-3 binding protein10aplasma-derived extracellular vesicles10aproteomics1 aRana, Rashmi1 aChauhan, Kirti1 aGautam, Poonam1 aKulkarni, Mahesh1 aBanarjee, Reema1 aChugh, Parul1 aChhabra, Satnam, Singh1 aAcharya, Rajesh1 aKalra, Samir, Kumar1 aGupta, Anshul1 aJain, Sunila1 aGanguly, Nirmal, Kumar  uhttp://library.ncl.res.in/content/plasma-derived-extracellular-vesicles-reveal-galectin-3-binding-protein-potential-biomarker