02573nas a2200325   4500008004100000022001400041245010700055210007100162260000900233300001400242490000700256520148700263653003201750653001501782653004001797653003001837653001801867653001701885100002501902700001901927700002101946700002301967700001801990700001402008700002102022700001902043700002502062700002102087856013902108       2025                        eng d  a1756-891900aEffectiveness of 3-amino-2-thiocyanato-α, β-unsaturated carbonyl compounds against chikungunya virus0 aEffectiveness of 3amino2thiocyanatoα βunsaturated carbonyl compo  cJUN   a1269-12790 v173 a<p>Aim Chikungunya fever (CHIKF) caused by the chikungunya virus (CHIKV) is characterized by the presence of long-term polyarthralgia in a minor proportion of the infected patients. Currently, there are no FDA-approved antivirals available. This study evaluated the anti-CHIKV potential of 16 synthetic 3-amino-2-thiocyanato-alpha, beta-unsaturated carbonyl compounds and elucidated their probable mechanisms of action. Methods Anti-CHIKV activity of 16 compounds were investigated in Vero CCL-81 cells using focus forming unit assay (FFU). Dose-dependent and time-dependent antiviral assays were performed for the effective compounds. Molecular docking was performed to find out their interactions with viral proteins. Results Five compounds showed promising anti-CHIKV activity by reducing viral titer with &gt;1 log10 FFU/ml. Dose-dependent studies revealed that the compound 3 g was more effective in reducing the virus titer with a half-maximal inhibitory concentration (IC50) of 0.4315 mu M and a selectivity index of 35.99. Docking analyses revealed that all the compounds mainly interact with the non-structural proteins of CHIKV. Conclusions These findings demonstrate the in vitro anti-CHIKV activity of these compounds, and their possible mode of action via interference with early stages of infection and replication processes. This study warrants further preclinical and clinical evaluation to establish their safety and efficacy as novel anti-CHIKV therapeutics.</p>
10a3-amino-2-thiocyanato-alpha10aantivirals10abeta-unsaturated carbonyl compounds10aChikungunya virus (CHIKV)10ansP2 helicase10atherapeutics1 aAlagarasu, Kalichamy1 aDhote, Radhika1 aBagad, Pooja, K.1 aKharikar, Dwidhesh1 aPatil, Poonam1 aRoy, Diya1 aShukla, Shridhar1 aCherian, Sarah1 aSenthilkumar, Beeran1 aParashar, Deepti  uhttp://library.ncl.res.in/content/effectiveness-3-amino-2-thiocyanato-%CE%B1-%CE%B2-unsaturated-carbonyl-compounds-against-chikungunya