02321nas a2200253   4500008004100000022001400041245014300055210006900198260000800267490000800275520140100283653001701684653003701701653001401738653002501752653001901777100002301796700003201819700002101851700002501872700002201897700002001919856012801939       2024                        eng d  a0965-174800aAminoacylase efficiently hydrolyses fatty acid amino acid conjugates of Helicoverpa armigera potentially to increase the pool of glutamine0 aAminoacylase efficiently hydrolyses fatty acid amino acid conjug  cFEB0 v1653 a<p>
	One of the most prevalent bioactive molecules present in the oral secretion (OS) of lepidopteran insects is fatty acid amino acid conjugates (FACs). Insect dietary components have influence on the synthesis and retaining the pool of FACs in the OS. We noted differential and diet-specific accumulation of FACs in the OS of Helicoverpa armigera by using Liquid Chromatography-Quadrupole Time of Flight Mass Spectrometry. Interestingly, we identified FACs hydrolyzing enzyme aminoacylase (HaACY) in the OS of H. armigera through proteomic analysis. Next, we have cloned, expressed, and purified active recombinant HaACY in the bacterial system. Recombinant HaACY hydrolyzes all the six identified FACs in the OS of H. armigera larvae fed on host and non-host plants and releases respective fatty acid and glutamine. In these six FACs, fatty acid moieties vary while amino acid glutamine was common. Glutamine obtained upon hydrolysis of FACs by HaACY might serve as an amino acid pool for insect growth and development. To understand the substrate choices of HaACY, we chemically synthesized, purified, and characterized all the six FACs. Interestingly, rHaACY also shows hydrolysis of synthetic FACs into respective fatty acid and glutamine. Our results underline the importance of diet on accumulation of FACs and role of aminoacylase(s) in regulating the level of FACs and glutamine.</p>
10aAminoacylase10aFatty acid amino acid conjugates10aGlutamine10aHelicoverpa armigera10aOral secretion1 aKallure, Gopal, S.1 aSahoo, Shubhranshu, Shekhar1 aKale, Rutuja, S.1 aBarvkar, Vitthal, T.1 aKontham, Ravindar1 aGiri, Ashok, P.  uhttp://library.ncl.res.in/content/aminoacylase-efficiently-hydrolyses-fatty-acid-amino-acid-conjugates-helicoverpa-armigera03080nas a2200325   4500008004100000022001400041245012700055210006900182260000800251300001200259490000800271520196900279653002702248653004002275653002202315653002002337653001502357653001702372653002902389653002902418653001302447100002502460700002902485700003202514700002502546700002102571700002202592700002002614856012002634       2024                        eng d  a0960-741200aOcimum kilimandscharicum 4CL11 negatively regulates adventitious root development via accumulation of flavonoid glycosides0 aOcimum kilimandscharicum 4CL11 negatively regulates adventitious  cJUL  a176-1960 v1193 a<p>
	4-Coumarate-CoA Ligase (4CL) is an important enzyme in the phenylpropanoid biosynthesis pathway. Multiple 4CLs are identified in Ocimum species; however, their in planta functions remain enigmatic. In this study, we independently overexpressed three Ok4CL isoforms from Ocimum kilimandscharicum (Ok4CL7, -11, and -15) in Nicotiana benthamiana. Interestingly, Ok4CL11 overexpression (OE) caused a rootless or reduced root growth phenotype, whereas overexpression of Ok4CL15 produced normal adventitious root (AR) growth. Ok4CL11 overexpression in N. benthamiana resulted in upregulation of genes involved in flavonoid biosynthesis and associated glycosyltransferases accompanied by accumulation of specific flavonoid-glycosides (kaempferol-3-rhamnoside, kaempferol-3,7-O-bis-alpha-l-rhamnoside [K3,7R], and quercetin-3-O-rutinoside) that possibly reduced auxin levels in plants, and such effects were not seen for Ok4CL7 and -15. Docking analysis suggested that auxin transporters (PINs/LAXs) have higher binding affinity to these specific flavonoid-glycosides, and thus could disrupt auxin transport/signaling, which cumulatively resulted in a rootless phenotype. Reduced auxin levels, increased K3,7R in the middle and basal stem sections, and grafting experiments (intra and inter-species) indicated a disruption of auxin transport by K3,7R and its negative effect on AR development. Supplementation of flavonoids and the specific glycosides accumulated by Ok4CL11-OE to the wild-type N. benthamiana explants delayed the AR emergence and also inhibited AR growth. While overexpression of all three Ok4CLs increased lignin accumulation, flavonoids, and their specific glycosides were accumulated only in Ok4CL11-OE lines. In summary, our study reveals unique indirect function of Ok4CL11 to increase specific flavonoids and their glycosides, which are negative regulators of root growth, likely involved in inhibition of auxin transport and signaling.</p>
10a4-Coumarate-CoA ligase10a7-O-bis-alpha-<sc>l</sc>-rhamnoside10aadventitious root10aauxin transport10aFlavonoids10akaempferol-310aOcimum kilimandscharicum10aquercetin-3-O-rutinoside10arootless1 aLavhale, Santosh, G.1 aKondhare, Kirtikumar, R.1 aSinthadurai, Veenothini, S.1 aBarvkar, Vitthal, T.1 aKale, Rutuja, S.1 aJoshi, Rakesh, S.1 aGiri, Ashok, P.  uhttp://library.ncl.res.in/content/ocimum-kilimandscharicum-4cl11-negatively-regulates-adventitious-root-development