01568nas a2200145   4500008004100000245015300041210006900194260000800263300001600271490000700287520095300294100002901247700002201276856012401298       2022                        eng d00aPoly(N-vinylcaprolactam) containing solid lipid polymer hybrid nanoparticles for controlled delivery of a hydrophilic drug gemcitabine hydrochloride0 aPolyNvinylcaprolactam containing solid lipid polymer hybrid nano  cJUN  a17621-176280 v123 a<p>
	Folic acid tagged and hydrophilic polymer containing solid lipid nanoparticles (SLNs) were formulated for the controlled and targeted delivery of gemcitabine, a hydrophilic drug. Drug loaded SLNs were prepared by double emulsion method and optimized by 3(2) level factorial design. Then, a hydrophilic polymer, namely, poly(N-vinylcaprolactam) (PVCL) was incorporated in the optimized SLN batch in the first aqueous phase (W1) to obtain solid lipid polymer hybrid nanoparticles (SLPHNs) that were further decorated with folic acid (F-SLPHNs). TEM analysis of SLNs and SLPHNs revealed the spherical shape with no aggregation while SLPHNs showed higher % EE. SLPHNs exhibited limited burst release of gemcitabine compared to SLNs as well as lower overall % release. All the formulations showed good cytocompatibility against MDA-MB-231 cell lines and folic acid-tagged hybrid particles (F-SLPHNs) showed remarkably higher cellular uptake.</p>
1 aSurapaneni, Sai, Geetika1 aAmbade, Ashootosh  uhttp://library.ncl.res.in/content/polyn-vinylcaprolactam-containing-solid-lipid-polymer-hybrid-nanoparticles-controlled02222nas a2200241   4500008004100000245016000041210006900201260000800270300001100278490000800289520133700297653002101634653002301655653001801678653001601696653001701712653002301729100002901752700002601781700002601807700002601833856012101859       2023                        eng d00aPermeable polymersomes from temperature and pH dual stimuli-responsive PVCL-b-PLL block copolymers for enhanced cell internalization and lysosome targeting0 aPermeable polymersomes from temperature and pH dual stimulirespo  cAUG  a2134540 v1513 a<p>
	A series of dual stimuli-responsive block copolymers comprising temperature-responsive poly(N-vinyl-caprolactam) (PVCL) and biodegradable pH-responsive poly(L-lysine) (PLL) of varying chain length were syn-thesized by a combination of free radical polymerization and ring opening polymerization. The block copolymers formed micelles and vesicles (polymersomes) in response to temperature and pH, respectively, in aqueous so-lution. The nanoassemblies were characterized by transmission electron microscopy and dynamic light scattering techniques. Encapsulation of both hydrophobic and hydrophilic dyes in the polymersomes was shown. Doxo-rubicin (DOX) was loaded in the polymersomes and its controlled release in response to the two stimuli, inde-pendently and jointly, was studied. The drug was found to be released due to stimuli-induced increased permeability without disassembly of the polymersomes. A significant increase in the cellular uptake of the drug-loaded polymersomes at hyperthermia conditions was demonstrated at 41 degrees C and release of the drug upon localization in lysosomes was observed. Cellular internalization pathway of the polymersomes was investigated by competitive inhibition assay and a combination of endocytic pathways dominated by caveolae-mediated mechanism was found to be operative.</p>
10ablock copolymers10aControlled release10adrug delivery10aendocytosis10aPolymersomes10aStimuli-responsive1 aSurapaneni, Sai, Geetika1 aChoudhari, Shakeb, N.1 aAvhad, Shankarrao, V.1 aAmbade, Ashootosh, V.  uhttp://library.ncl.res.in/content/permeable-polymersomes-temperature-and-ph-dual-stimuli-responsive-pvcl-b-pll-block