02458nas a2200277   4500008004100000020001300041245007900054210006900133260000800202300000900210490000700219520159500226653002501821653002001846653001401866653003001880653002601910653002601936100002201962700002001984700001802004700002202022700002102044700001702065856009802082       2021                        eng d  a0971119800aGreen antibacterial molecules: sophorolipids with varying fatty acid chain0 aGreen antibacterial molecules sophorolipids with varying fatty a  cNOV  a431+0 v353 aAntibiotic resistance is a rising problem of over the last decade making it difficult to treat infections caused by various pathogenic bacteria. To tackle this problem, newer antimicrobial strategies are being explored. Sophorolipids (SLs) are a class of biomolecules produced by a non-pathogenic yeast Starmarella bombicola (S. bombicold) with surfactant as well as potent broad spectrum antibacterial activity. In this work, we have successfully synthesized sophorolipids using different fatty acids with increasing chain length of hydrophobic tail; Lauric acid (C12)-LASL, Myristic acid (C14)-MASL, Palmitic acid (C16)-PASL and Stearic acid (C18)-SASL. We have used various techniques like FTIR, HRMS to characterize the synthesized SL. Further, we have measured properties like the critical micellar concentration for these biosurfactants and the SL's showed CMC less than or equal to 100 mg/L indicating excellent surface-active property. We have studied the antibacterial activity against gram-negative and gram-positive bacteria. Interestingly, the antibacterial activity was found to be more potent as the chain length of the fatty acid in the SL decreased. This trend was found to be reverse for toxicity towards mammalian cells. The shorter chain fatty acid SL's were found to be less cytotoxic. These results indicate that the sophorolipid candidates with shorter chain lengths would be beneficial for various biomedical applications such as tissue engineering, regenerative medicine and wound healing. Keywords: sophorolipid, biosurfactant, short chain fatty acid, antibacterial10aAntibacterial agents10aCells (Biology)10aInfection10aMicrobial drug resistance10aSaturated fatty acids10aSurface active agents1 aHirlekar, Swarali1 aAbhyankar, Isha1 aKane, Kartiki1 aTrimukhe, Kalpana1 aPrabhune, Asmita1 aNisal, Anuya  uhttps://link.gale.com/apps/doc/A684660702/AONE?u=anon~d0cd1b4e&sid=googleScholar&xid=1a6d61d902197nas a2200241   4500008004100000245009300041210006900134260000800203300001100211490000700222520140600229653001701635653002101652653002301673653002401696653001701720100002101737700001801758700002001776700001701796700001901813856012301832       2022                        eng d00aSilk fibroin and recombinant elastin blend nano-coatings for implantable medical devices0 aSilk fibroin and recombinant elastin blend nanocoatings for impl  cDEC  a1048750 v333 a<p>
	Silicone breast implants are conventionally used to reconstruct the shape and size of the breast in breast cancer patients. However, rate of failure of implants due to formation of capsular contracture is significantly high. Recent studies have shown that coating the surface of the implant with a biocompatible polymer can mitigate this risk. Here, we have modified the surface of implant using a biocompatible natural biopolymer silk fibroin (SF). Further, we have developed formulations of SF with a recombinantly produced elastin-like-peptide (ELP) and compared their performance with a known anti-fouling hydrophilic polymer - polyethylene oxide (PEO). Microscopic and spectroscopic characterization confirm the formation of uniform coatings. These coatings have been also characterized for their ability to resists crack formation. Further, the coatings have been evaluated for their biological performance. Our studies show that addition of 25 wt% of ELP to SF significantly enhances the crack resistance for the coatings. In addition, SF/ELP coatings reduce the adsorption of blood plasma proteins by more than 80 %. Preliminary analysis shows that the SF/ELP blend coatings are non-cytotoxic and support adhesion, growth and proliferation of fibroblast cells. The study therefore demonstrates that SF/ELP coatings have the potential to mitigate the risk of breast implant failure.</p>
10aBlood plasma10aCrack resistance10aProtein adsorption10aRecombinant elastin10aSilk fibroin1 aJoseph, Emmanuel1 aKane, Kartiki1 aParekh, Nimisha1 aNisal, Anuya1 aJanorkar, Amol  uhttp://library.ncl.res.in/content/silk-fibroin-and-recombinant-elastin-blend-nano-coatings-implantable-medical-devices02284nas a2200277   4500008004100000022001400041245011300055210006900168260000800237300001200245490000800257520137100265653002801636653002101664653001001685653002801695653002501723100002001748700001901768700001801787700002001805700001701825700002201842700002001864856012201884       2022                        eng d  a0141-813000aSuperior processability of Antheraea mylitta silk with cryo-milling: performance in bone tissue regeneration0 aSuperior processability of Antheraea mylitta silk with cryomilli  cJUL  a155-1650 v2133 a<p>
	Non-mulberry silk polymers have a promising future in biomedical applications. However, the dissolution of nonmulberry silk fiber is a still challenge and this poor processability has limited the use of this material. Here, we report a unique protocol to process the Antheraea mylitta (AM) silk fiber. We have shown that the cryo-milling of silk fiber reduces the beta sheet content by more than 10% and results in an SF powder that completely dissolves in routine solvents like trifluoroacetic acid (TFA) within few hours to form highly concentrated solutions (\~20 wt %). Further, these solutions can be processed using conventional processing techniques such as electrospinning to form 3D scaffolds. Bombyx mori (BM) silk was used as a control sample in the study. In-vitro studies were also performed to monitor cell adhesion and proliferation and hMSCs differentiation into osteogenic lineage. Finally, the osteogenic potential of the scaffolds was also evaluated by a 4-week implantation study in rat calvarial model. The in-vitro and in-vivo results show that the processing techniques do not affect the biocompatibility of the material and the AM scaffolds support bone regeneration. Our results, thus, show that cryo-milling facilitates enhanced processability of non-mulberry silk and therefore expands its potential in biomedical applications.</p>
10aBone tissue engineering10aCalvarial defect10ahMSCs10aNon-mulberry silk fiber10aProcessing technique1 aParekh, Nimisha1 aBijosh, C., K.1 aKane, Kartiki1 aPanicker, Alaka1 aNisal, Anuya1 aWangikar, Pralhad1 aAgawane, Sachin  uhttp://library.ncl.res.in/content/superior-processability-antheraea-mylitta-silk-cryo-milling-performance-bone-tissue