02846nas a2200289   4500008004100000022001400041245012500055210006900180260000800249300001100257490000700268520189300275653001702168653001102185653001402196653001502210653003102225100001502256700003102271700002102302700002702323700002402350700001702374700002102391700001702412856012702429       2021                        eng d  a1773-224700aBiological synthesis of alpha-Ag2S composite nanoparticles using the fungus Humicola sp. and its biomedical applications0 aBiological synthesis of alphaAg2S composite nanoparticles using   cDEC  a1027700 v663 aSynthesis of alpha-Ag2S (silver sulfide) is a highly challenging task, usually attempted using synthetic, hazardous chemical processes. This report puts efforts to exploring microbial-based eco-friendly alternatives. A facile, onepot biological method for the synthesis of alpha-Ag2S composite nanoparticles (NPs) in an aqueous medium at ambient conditions was established. As prepared alpha-Ag2S composite NPs were efficiently produced from precursors silver nitrate and sodium sulfite. The extracellular biomolecules produced by Humicola sp. reduced the precursor solution and at the same time it capped the formed Ag2S NPs. The characterization performed by different techniques revealed excellent optical, morphological, and structural properties (size, shape distribution, and crystallinity) of the alpha-Ag2S NPs. The change in color from colorless to dark brown indicated the formation of alpha-Ag2S, which was further confirmed by fluorescence and UV-Visible spectroscopy, showing a sharp peak at 410 nm. Morphological analysis of alpha-Ag2S NPs confirmed the spherical shape, and narrow size distribution. alpha-Ag2S NPs were found to be potent in antimicrobial applications owing to their high dispersibility in water and capping by extracellular biomolecules secreted by Humicola sp. The anti-cancer activity for alpha-Ag2S performed using human breast cancer cell line (ZR-75-1) and human Burkitt's lymphoma cancer (Daudi). The biocompatibility test was performed using human peripheral blood mononuclear cells (PBMCs) for alpha-Ag2S NPs. The anti-leishmania activity was also determined for alpha Ag2S NPs in-vitro using the standard anti-promastigote protocol (Leishmania donovani promastigotes). This extracellular system is an economically and environmentally benign route for the biosynthesis of alpha-Ag2S NPs having desired surface, structure and biomedical properties.10aCytotoxicity10aFungus10aHemolysis10aHumicolasp10aSilver sulfide(alpha-Ag2S)1 aSyed, Asad1 aSaedi, Marzouq, Hussain Al1 aBahkali, Ali, H.1 aElgorban, Abdallah, M.1 aKharat, Maheshkumar1 aPai, Kalpana1 aGhodake, Gajanan1 aAhmad, Absar  uhttp://library.ncl.res.in/content/biological-synthesis-alpha-ag2s-composite-nanoparticles-using-fungus-humicola-sp-and-its