01440nas a2200193 4500008004100000022001400041245010300055210006900158260012500227300002300352490000700375520064400382100002901026700001701055700001701072700002001089700001301109856012401122 2006 eng d a0003-695100aPhysical manipulation of biological and chemical syntheses for nanoparticle shape and size control0 aPhysical manipulation of biological and chemical syntheses for n aCIRCULATION & FULFILLMENT DIV, 2 HUNTINGTON QUADRANGLE, STE 1 N O 1, MELVILLE, NY 11747-4501 USAbAMER INST PHYSICScDEC aArticle No. 2631050 v893 a

A nanosynthesis scheme is demonstrated which renders excellent control of nanoparticle shape, size, and dispersity in a solution based synthesis process. The scheme, termed as percolative microcavity synthesis, involves the use of a granular medium with percolative microcavities which facilitate nearly similar grain size/shape dependent reaction zones limiting intrinsic growth inhomogeneities, enabling particle size/shape control. The viability of the process is demonstrated for the synthesis of gold nanoparticles by a plant extract based biological method as well as a chemical method. (c) 2006 American Institute of Physics.

1 aOgale, Satishchandra, B.1 aAhmad, Absar1 aPasricha, R.1 aDhas, Vivek, V.1 aSyed, A. uhttp://library.ncl.res.in/content/physical-manipulation-biological-and-chemical-syntheses-nanoparticle-shape-and-size-101804nas a2200241 4500008004100000022001400041245012200055210006900177260007900246300001200325490000800337520092300345653002201268653002001290653001101310653001701321653000801338653002901346100001601375700001701391700002601408856012801434 2007 eng d a0003-986100aPurification and characterization of a lectin from endophytic fungus fusarium solani having complex sugar specificity0 aPurification and characterization of a lectin from endophytic fu a360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USAbELSEVIER SCIENCE INCcJAN a243-2510 v4573 a

A lectin from the mycelial extract of an endophytic strain of Fusarium solani was purified. Its hemagglutinating activity was inhibited by glycoproteins possessing N-linked as well as O-linked glycans. The thermodynamics and kinetics of binding of glycans and glycoproteins to F. solani lectin Was Studied using SUH ace plasmon resonance. The lectin showed high affinity for asialofetuin, asia-lofibrinogen, asialofibrinogen, and thyroglobulin: and comparatively low affinity for mucin, fetuin, fibrinogen, and holotransferrin. Glycoproteins showed glycans with significant contribution from enthalpy and positive entropy, suggesting several fold higher affinity than their corresponding g the involvement of non-polar protein-protein interaction. Moreover, the higher affinity of the glycoproteins was due to their faster association rates and low activation energy. (c) 2006 Elsevier Inc. All rights reserved.

10aendophytic fungus10aFusarium solani10aLectin10aPurification10aSPR10aThermodynamic properties1 aKhan, Feroz1 aAhmad, Absar1 aKhan, Mohammad, Islam uhttp://library.ncl.res.in/content/purification-and-characterization-lectin-endophytic-fungus-fusarium-solani-having-complex01688nas a2200277 4500008004100000022001400041245016700055210006900222260014100291300001400432490000700446520061700453653001501070653002101085653002001106653001001126653001801136100001801154700001301172700001501185700001901200700002101219700001701240700002601257856012701283 2009 eng d a1017-782500aProduction, purification, and characterization of taxol and 10-DABIII from a new endophytic fungus gliocladium sp isolated from the Indian yew tree, taxus baccata0 aProduction purification and characterization of taxol and 10DABI aKOREA SCI TECHNOL CENTER \#507, 635-4 YEOGSAM-DONG, KANGNAM-GU, SEOUL 135-703, SOUTH KOREAbKOREAN SOC MICROBIOLOGY & BIOTECHNOLOGYcNOV a1342-13470 v193 a

We have isolated endophytic fungi from the Indian yew tree, Taxus baccata, and then screened for taxol production. Out of the 40 fungal cultures screened, one fungus Gliocladium sp. was found to produce taxol and 10-DABIII (10-deacetyl baccatin III). These compounds were purified by TLC and HPLC and characterized using UV-spectroscopy, ESI-MS, MS/MS, and proton NMR. One liter of Gliocladium sp. culture yielded 10 mu g of taxol and 65 mu g of 10-DABIII. The purified taxol from the fungus showed cytotoxicity towards cancer lines HL-60 (leukemia), A431 (epidermal carcinoma), and MCF-7 (breast cancer).

10a10 DAB III10aEndophytic fungi10aGliocladium sp.10aTaxol10aTaxus baccata1 aSreekanth, D.1 aSyed, A.1 aSarkar, S.1 aSarkar, Dhiman1 aSanthakumari, B.1 aAhmad, Absar1 aKhan, Mohammad, Islam uhttp://library.ncl.res.in/content/production-purification-and-characterization-taxol-and-10-dabiii-new-endophytic-fungus-002295nas a2200241 4500008004100000022001400041245011800055210006900173260006700242300001200309490000700321520147000328653001001798653001801808653001701826653001101843653000901854653000801863100001801871700002101889700001701910856012601927 2012 eng d a0885-451300aPhytosynthesis of intracellular and extracellular gold nanoparticles by living peanut plant (Arachis hypogaea L.)0 aPhytosynthesis of intracellular and extracellular gold nanoparti a111 RIVER ST, HOBOKEN 07030-5774, NJ USAbWILEY-BLACKWELLcNOV a471-4780 v593 a

Inorganic nanomaterials of different chemical compositions are conventionally synthesized under harsh environments such as extremes of temperature, pressure, and pH. Moreover, these methods are eco-unfriendly and cumbersome, yield bigger particles, and agglomerate because of not being capped by capping agents. In contrast, biological synthesis of inorganic nanomaterials occurs under ambient conditions, namely room temperature, atmospheric pressure, and physiological pH. These methods are reliable, eco-friendly, and cheap. In this paper, we report for the first time the extracellular and intracellular synthesis of gold nanoparticles (GNPs) using living peanut seedlings. The formed GNPs were highly stable in solution and inside the plant tissue. Transmission electron microscopy revealed that extracellular GNPs distributions were in the form of monodispersed nanoparticles. The nanoparticles ranged from 4 to 6 nm in size. The intercellular nanoparticles were of oval shape and size ranged from 5 to 50 nm. Both extracellular and intracellular nanoparticles were further characterized by standard techniques. The formed GNPs inside the plant tissue were estimated by inductively coupled plasma spectrometry. This opens up an exciting possibility of a plant-based nanoparticle synthesis strategy, wherein the nanoparticles may be entrapped in the biomass in the form of a film or produced in the solution, both of which have interesting applications.

10acells10aextracellular10aliving plant10aPeanut10aroot10aTEM1 aRaju, Dugyala1 aMehta, Urmil, J.1 aAhmad, Absar uhttp://library.ncl.res.in/content/phytosynthesis-intracellular-and-extracellular-gold-nanoparticles-living-peanut-plant-001841nas a2200217 4500008004100000022001400041245008200055210006900137260006900206300001200275490000800287520110600295653001201401653002201413653001301435653001601448653000901464100002201473700001701495856011101512 2013 eng d a1385-894700aPhase, size and shape transformation by fungal biotransformation of bulk TiO20 aPhase size and shape transformation by fungal biotransformation  aPO BOX 564, 1001 LAUSANNE, SWITZERLANDbELSEVIER SCIENCE SAcAUG a367-3710 v2303 a

Nanosynthesis is an ever expanding frontier in recent years in view of its implications to many future technologies covering diverse fields. Considerable progress has been made realizing high quality synthesis of elemental nanomaterials as well as compounds as chemical, biological and physical routes. The issue of technologically viable large scale synthesis still continues to be a challenge. Here we demonstrate a novel environmentally friendly top down approach to nanosynthesis which exploits the strength and peculiarities of fungus based bioleaching in extracting radicals from compounds and then providing them with a reactive as well as capping environment. Thus protein capped nanoparticles of TiO2 (5-28 nm, circular and brookite phase) are formed directly from micron size (150-250 nm, disc shape, anatase phase) powder by exposing them to a medium of fungus Humicola sp. at just 50 degrees C. The fungus Humicola sp. is not only useful for the synthesis of nanoparticles but also for the transformation of shape, phase and size of TiO2. (C) 2013 Elsevier B.V. All rights reserved.

10aAnatase10aBiotransformation10aBrookite10ahumicola sp10aTiO21 aKhan, Shadab, Ali1 aAhmad, Absar uhttp://library.ncl.res.in/content/phase-size-and-shape-transformation-fungal-biotransformation-bulk-tio2-001812nas a2200157 4500008004100000245008600041210006900127260000900196300001600205490000600221520122900227100002901456700001701485700003601502856011601538 2019 eng d00aProtein-capped metal nanoparticles inhibit tau aggregation in alzheimer's disease0 aProteincapped metal nanoparticles inhibit tau aggregation in alz cJULY a12833-128400 v43 a

The Alzheimer's disease (AD) therapeutic research is yielding a large number of potent molecules. The nanoparticle-based therapeutics against the protein aggregation in AD is also taking a lead especially with amyloid-beta as a primary target. In this work, we have screened for the first time protein-capped (PC) metal nanoparticles for their potency in inhibiting Tau aggregation in vitro. We present a novel function of PC-Fe3O4 and PC-CdS nanoparticles as potent Tau aggregation inhibitors by fluorescence spectrometry, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and electron microscopy. We demonstrate that the biologically synthesized PC-metal nanoparticles, especially iron oxide do not affect the viability of neuroblastoma cells. Moreover, PC-CdS nanoparticles show dual properties of inhibition and disaggregation of Tau. Thus, the nanoparticles can take a lead as potent Tau aggregation inhibitors and can be modified for specific drug delivery due to their very small size. The current work presents unprecedented strategy to design anti-Tau aggregation drugs, which provides interesting insights to understand the role of biological nanostructures in Alzheimer's disease.
 

1 aSonawane, Shweta, Kishor1 aAhmad, Absar1 aChinnathambi, Subashchandrabose uhttp://library.ncl.res.in/content/protein-capped-metal-nanoparticles-inhibit-tau-aggregation-alzheimers-disease