02021nas a2200253   4500008004100000022001400041245010800055210006900163260000900232300001100241490000700252520118300259653002301442653002901465653001501494653002601509653001401535100002001549700001701569700001901586700002001605700002001625856012201645       2020                        eng d  a0734-975000aEngineering staphylococcal protein A for high-throughput affinity purification of monoclonal antibodies0 aEngineering staphylococcal protein A for highthroughput affinity  cNOV   a1076320 v443 a<p>Protein A chromatography is one of the most widely used purification steps in the manufacturing of the various classes of recombinant and non-recombinant antibodies. Due to the higher cost, lower binding capacity, and limited life cycle of Protein A ligand, this affinity-based purification step is often one of the most significant contributors to the cost of manufacturing of monoclonal antibody (mAb) products. In the last decade, there has been significant progress in improving the Protein A chromatography throughput by designing new engineered Staphylococcal Protein A (SPA) variants with higher dynamic binding capacity, considerable alkaline tolerance, and mild acidic elution pH. This review aims at summarizing the various protein engineering approaches used for improving the throughput of the Protein A-based affinity purification of various immunoglobulins. With biopharmaceutical producers operating under ever-increasing pressure towards reducing the cost of manufacturing, these advances in engineered protein A variants will help in processing larger cell culture volumes with high throughput and thereby significantly lower the cost of raw materials.</p>
10aAlkaline tolerance10aDynamic binding capacity10aElution pH10aMonoclonal antibodies10aProtein A1 aAmritkar, Vinod1 aAdat, Satish1 aTejwani, Vijay1 aRathore, Anurag1 aBhambure, Rahul  uhttp://library.ncl.res.in/content/engineering-staphylococcal-protein-high-throughput-affinity-purification-monoclonal