02149nas a2200253   4500008004100000022001400041245011200055210006900167260005700236300001400293490000700307520127000314653002801584653002401612653000901636653001301645653002101658100001501679700001901694700001801713700002001731700002401751856012001775       2013                        eng d  a0959-399300aProduction, purification and characterization of cholesterol oxidase from a newly isolated streptomyces sp.0 aProduction purification and characterization of cholesterol oxid  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcNOV  a2077-20850 v293 a<p>Cholesterol oxidase production (COD) by a new isolate characterized as Streptomyces sp. was studied in different production media and fermentation conditions. Individual supplementation of 1 % maltose, lactose, sucrose, peptone, soybean meal and yeast extract enhanced COD production by 80-110 % in comparison to the basal production medium (2.4 U/ml). Supplementation of 0.05 % cholesterol (inducer) enhanced COD production by 150 %. COD was purified 14.3-fold and its molecular weight was found to be 62 kDa. V-max (21.93 mu M/min mg) and substrate affinity K-m (101.3 mu M) suggested high affinity of the COD for cholesterol. In presence of Ba2+ and Hg2+ the enzyme activity was inhibited while Cu2+ enhanced the activity nearly threefold. Relative activity of the enzyme was found maximum in triton X-100 whereas sodium dodecyl sulfate inactivated the enzyme. The enzyme activity was also inhibited by the thiol-reducing reagents like Dithiothreitol and beta-mercaptoethanol. The COD showed moderate stability towards all organic solvents except acetone, benzene and chloroform. The activity increased in presence of isopropanol and ethanol. The enzyme was most active at pH 7 and 37 A degrees C temperature. This organism is not reported to produce COD.</p>10aAffinity chromatography10aCholesterol oxidase10aHPLC10aSDS PAGE10aStreptomyces sp.1 aNiwas, Ram1 aSingh, Vineeta1 aSingh, Rajbir1 aTripathi, Divya1 aTripathi, C., K. M.  uhttp://library.ncl.res.in/content/production-purification-and-characterization-cholesterol-oxidase-newly-isolated-002383nas a2200217   4500008004100000022001400041245011100055210006900166260009000235300002600325490000600351520153400357100001901891700002101910700002001931700001901951700002301970700001801993700002402011856013002035       2015                        eng d  a2045-232200aIsolation, characterization and antifungal docking studies of wortmannin isolated from penicillium radicum0 aIsolation characterization and antifungal docking studies of wor  aMACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLANDbNATURE PUBLISHING GROUPcJUL  aArticle Number: 119480 v53 a<p>During the search for a potent antifungal drug, a cell-permeable metabolite was isolated from a soil isolate taxonomically identified as Penicillium radicum. The strain was found to be a potent antifungal agent. Production conditions of the active compound were optimized and the active compound was isolated, purified, characterized and identified as a phosphoinositide 3-kinase (PI3K) inhibitor, commonly known as wortmannin (Wtmn). This is very first time we are reporting the production of Wtmn from P. radicum. In addition to its previously discovered anticancer properties, the broad spectrum antifungal property of Wtmn was re-confirmed using various fungal strains. Virtual screening was performed through molecular docking studies against potential antifungal targets, and it was found that Wtmn was predicted to impede the actions of these targets more efficiently than known antifungal compounds such as voriconazole and nikkomycin i.e. 1) mevalonate-5-diphosphate decarboxylase (1FI4), responsible for sterol/isoprenoid biosynthesis; 2) exocyst complex component SEC3 (3A58) where Rho-and phosphoinositide-dependent localization is present and 3) Kre2p/Mnt1p a Golgi alpha1,2-mannosyltransferase (1S4N) involved in the biosynthesis of yeast cell wall glycoproteins). We conclude that Wtmn produced from P. radicum is a promising lead compound which could be potentially used as an efficient antifungal drug in the near future after appropriate structural modifications to reduce toxicity and improve stability.</p>1 aSingh, Vineeta1 aPraveen, Vandana1 aTripathi, Divya1 aHaque, Shafiul1 aSomvanshi, Pallavi1 aKatti, S., B.1 aTripathi, C., K. M.  uhttp://library.ncl.res.in/content/isolation-characterization-and-antifungal-docking-studies-wortmannin-isolated-penicillium-1