02405nas a2200289 4500008004100000022001400041245015700055210006900212260000800281300000900289490000600298520137000304100002201674700002701696700001901723700001901742700003101761700003601792700001801828700002001846700002301866700002101889700003401910700001601944700003301960856012201993 2018 eng d a2045-232200aStreptomyces sp metabolite(s) promotes Bax mediated intrinsic apoptosis and autophagy involving inhibition of mTOR pathway in cervical cancer cell lines0 aStreptomyces sp metabolites promotes Bax mediated intrinsic apop cFEB a28100 v83 a

In cervical cancer, the association between HPV infection and dysregulation of phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway (PI3K/AKT/mTOR pathway) places mTOR as an attractive therapeutic target. The failure of current treatment modalities in advanced stages of this cancer and drawbacks of already available mTOR inhibitors demand for novel drug candidates. In the present study we identified the presence of a mTOR inhibitor in an active fraction of the ethyl acetate extract of Streptomyces sp OA293. The metabolites(s) in the active fraction completely inhibited mTORC1 and thereby suppressed activation of both of its downstream targets, 4E-BP1 and P70S6k, in cervical cancer cells. In addition, it also stalled Akt activation via inhibition of mTORC2. The mechanism of mTOR inhibition detailed in our study overcomes significant drawbacks of well known mTOR inhibitors such as rapamycin and rapalogs. The active fraction induced autophagy and Bax mediated apoptosis suggesting that mTOR inhibition resulted in programmed cell death of cancer cells. The molecular weight determination of the components in active fraction confirmed the absence of any previously known natural mTOR inhibitor. This is the first report of complete mTOR complex inhibition by a product derived from microbial source.

1 aDan, Vipin, Mohan1 aMuralikrishnan, Balaji1 aSanawar, Rahul1 aVinodh, J., S.1 aBurkul, Bhushan, Bapusaheb1 aSrinivas, Kalanghad, Puthankala1 aLekshmi, Asha1 aPradeep, N., S.1 aDastager, Syed, G.1 aSanthakumari, B.1 aSanthoshkumar, Thankayyan, R.1 aKumar, Ajay1 aPillai, Madhavan, Radhakrish uhttp://library.ncl.res.in/content/streptomyces-sp-metabolites-promotes-bax-mediated-intrinsic-apoptosis-and-autophagy02296nas a2200229 4500008004100000022001400041245009200055210006900147260000800216300001600224490000700240520153400247100001401781700002201795700002601817700002401843700002301867700001901890700002301909700001501932856011901947 2020 eng d a0957-452200aStructural and electrical characterization studies for ternary composite of polypyrrole0 aStructural and electrical characterization studies for ternary c cNOV a18400-184110 v313 a

In this work, temperature dependence of alternate current (AC) and direct current (DC) conductivities of optimized polypyrrole/silver-tantalum oxide (PPy/Ag-Ta2O5), a ternary conducting polymer composite is comparatively studied with those of PPy and PPy/Ag. For the purpose, silver (Ag) nanoparticles were encapsulated with polypyrrole (PPy) by in situ oxidative polymerization to form core-shell structured PPy/Ag composite for which Ag nanoparticles were extracted from green tea. The PPy/Ag composite was then mechanically mixed with tantalum pentoxide (Ta2O5) to form PPy/Ag-Ta(2)O(5)ternary composite. Increase in depth of delocalization band of PPy in ternary composite as compared to those of PPy/Ag composite and PPy, indicating its increased AC conductivity confirmed from the comparative FTIR analyses. Interaction between PPy/Ag composite and Ta(2)O(5)in the ternary composite was confirmed from XRD studies. The formation of core-shell structured PPy/Ag composite and Ta(2)O(5)particles embedded in such PPy/Ag composite to form PPy/Ag-Ta(2)O(5)ternary composite confirmed from TEM and Raman studies. The frequency- and temperature-dependent electrical conductivity studies revealed increase in AC conductivity of the ternary composite as compared to those of PPy/Ag composite and pure PPy attributed mainly to interfacial effects. The charge transport in these samples predicted to be due to correlated barrier hopping of charges was confirmed by calculating their respective AC and DC activation energies.

1 aMegha, R.1 aRavikiran, Y., T.1 aKumari, S., C. Vijaya1 aPrakash, H., G. Raj1 aRevanasiddappa, M.1 aManjunatha, S.1 aDastager, Syed, G.1 aThomas, S. uhttp://library.ncl.res.in/content/structural-and-electrical-characterization-studies-ternary-composite-polypyrrole02654nas a2200241 4500008004100000022001400041245013500055210006900190260000800259300001200267490000700279520182500286653001502111653001702126653002402143653001302167653001702180653002302197100002202220700002302242700002302265856012402288 2024 eng d a1139-670900aStrain improvement for enhanced erythritol production by Moniliella pollinis Mutant-58 using jaggery as a cost-effective substrate0 aStrain improvement for enhanced erythritol production by Monilie cAPR a581-5960 v273 a

Erythritol has been produced by various microorganisms including Yarrowia, Moniliella, Aureobasidium, and Candida strains. Due to its relatively high price, erythritol sweetener is used lesser than other polyols despite having many advantages. Therefore, in this study, Moniliella pollinis strain was improved for erythritol production by chemical mutagenesis and subsequently screening for cost-effective carbon sources for the enhanced erythritol yield. M. pollinis was subjected to N-methyl N-nitro N-nitroso guanidine (NTG), ethyl methyl sulfonate (EMS), and UV mutagenesis for improved erythritol production. The fmutant strains were evaluated for enhanced erythritol production medium optimization by using different carbon substrates at the shake flask level. To enhance the production of erythritol and statistical media, optimization was carried out using a central composite design (CCD). Among 198 isolated mutants, Mutant-58 strain generated by EMS mutagenesis was selected for further assessment. The Mutant-58 strain showed significant morphological changes as compared to the parent strain. Furthermore, statistically optimized media composition resulted in the higher production of erythritol (91.2 & PLUSMN; 3.4 g/L) with a yield of 40.7 & PLUSMN; 3.4 % in shake flask experiments. The optimized medium composition for erythritol production constitutes (g/L) 225 jaggery, 4.4 yeast extract (YE), 4.4 KH2PO4, 0.31 MgSO4, and pH 5.5. The present study demonstrated strain improvement, media, and process optimization resulting in a 30% increase in the erythritol production in the Mutant-58 as compared to the parent strain. This is also the first instance where jaggery has been used as a cost-effective carbon source alternative to glucose for industrial-scale erythritol production.

10aErythritol10aFermentation10aMoniliella pollinis10aMutation10aOptimization10arenewable resource1 aKhatape, Anil, B.1 aRangaswamy, Vidhya1 aDastager, Syed, G. uhttp://library.ncl.res.in/content/strain-improvement-enhanced-erythritol-production-moniliella-pollinis-mutant-58-using