02273nas a2200169   4500008004100000245011600041210006900157260000800226300002300234490000600257520163200263100002001895700001601915700002301931700002201954856012701976       2017                        eng d00aPhytase production by aspergillus niger NCIM 563 for a novel application to degrade organophosphorus pesticides0 aPhytase production by aspergillus niger NCIM 563 for a novel app  cMAR  aArticle Number: 660 v73 aThe production of phytase using Aspergillus niger NCIM 563 under submerged fermentation conditions was studied using protein rich chickpea flour as substrate. Employing a hybrid statistical media optimization strategy of Plackett-Burman and Box-Behnken experimental designs in shake-flasks gave an increased phytase activity from an initial 66 IU/mL in 216 h to 160 IU/mL in a reduced time of 132 h. Productivity, thus increased by 3.97 times from 7.3 to 29 IU/mL/day. Using the optimized media, the production was successfully scaled-up further and improved up to 164 IU/mL in 96 h by studies carried out employing 2 and 10-L fermenters. The enzyme supernatant was recovered using centrifugal separation of biomass and the stability of the produced phytase was tested for animal feed applications under gastric conditions. In vitro degradation studies of water soluble monocrotophos, methyl parathion and water insoluble chlorpyrifos, pesticides used extensively in agriculture was carried out. It was observed by HPLC analysis that phytase could degrade 72% of chlorpyrifos at pH 7.0, 35 degrees C. Comparable results were obtained with monocrotophos and methyl parathion. With chlorpyrifos at higher temperature 50 degrees C as much as 91% degradation could be obtained. The degradation of chlorpyrifos was further validated by spraying phytase on harvested green chilli ( Capsicum annuum L) under normal conditions of pH 7.0, 35 degrees C and the degradation products obtained analyzed by LCMS. Thus, the present study brings out a potentially novel application of phytase for biodegradation of organophosphorus pesticides.1 aShah, Parin, C.1 aKumar, Ravi1 aDastager, Syed, G.1 aKhire, Jayant, M.  uhttp://library.ncl.res.in/content/phytase-production-aspergillus-niger-ncim-563-novel-application-degrade-organophosphorus02502nas a2200265   4500008004100000022001400041245013300055210006900188260000800257300001200265490000700277520158600284653002501870653001301895653001601908653001101924653002201935653001401957653004001971100002002011700002002031700002302051700004002074856012202114       2020                        eng d  a1867-130600aPlant probiotic bacterial endophyte, alcaligenes faecalis, modulates plant growth and forskolin biosynthesis incoleus forskohlii0 aPlant probiotic bacterial endophyte alcaligenes faecalis modulat  cJUN  a481-4930 v123 a<p>Coleus forskohliiis an herb, well-known for its medicinal compound forskolin present in its roots, with wide range of pharmaceutical applications. Here, we report, for the first time, the role of plant-probiotic bacterial endophytes ofC. forskohlii, CFLB1 and CFRB1, isolated from leaf and root, which regulate plant growth andin plantforskolin content. Native bacterial endophyte, CFRB1 (Alcaligenes faecalis), significantly modulates primary plant productivity and forskolin content under pot and field conditions. Under field conditions, CFRB1 endophyte application significantly enhanced photosynthetic pigments and reduced the severity of root-knot and root rot diseases. Expression analyses of functional genes involved in the forskolin biosynthesis inC. forskohliiplants treated with CFRB1 endophyte under field conditions revealed differential upregulation of fourC. forskohliiditerpene synthases (CfTPSs),CfTPS1,CfTPS2,CfTPS3andCfTPS4, along with cytochrome P450 (CfCYP76AH15) and acyltransferase (CfACT1-8) genes. CFRB1 treatment reduced the severity of nematode infection and root rot inC. forskohliiplants by 81 and 78%, respectively. Overall, we demonstrate that cross-talk of plant-endophyte interaction inC. forskohliiis beneficial, leading to enhanced forskolin content through modulation of forskolin biosynthetic pathway genes along with increased plant yield and reduced disease incidence. Thus, endophytic isolate,A. faecalis(CFRB1), could be deployed as a novel bio-stimulant for enhancingin plantaforskolin content during cultivation ofC. forskohlii.</p>
10aAlcaligenes faecalis10aCfACT1-810aCfCYP76AH1510aCfTPSs10aColeus forskohlii10aForskolin10aPlant-probiotic bacterial endophyte1 aMastan, Anthati1 aRane, Digeshwar1 aDastager, Syed, G.1 aBabu, Chikkarasanahalli, Shivegowda  uhttp://library.ncl.res.in/content/plant-probiotic-bacterial-endophyte-alcaligenes-faecalis-modulates-plant-growth-and02227nas a2200217   4500008004100000022001400041245006500055210006200120260000800182300001400190490000800204520159000212653002101802653001101823653001301834653001201847100002201859700001401881700002301895856009101918       2021                        eng d  a0302-893300aPriestia veravalensis sp. nov., isolated from coastal sample0 aPriestia veravalensis sp nov isolated from coastal sample  cOCT  a4839-48450 v2033 aThe taxonomic position of two isolates, SGD-V-76(T) and SGD-M-37, isolated from sediment sample of Veraval coast, India, was examined using the polyphasic taxonomic approach. The morphological and chemotaxonomic characteristics of these two organisms are typical of the genus Priestia. The phylogenetic analyses performed using almost complete 16S rRNA gene sequences demonstrated that the isolate belongs to the Bacillaceae family, and forms a clade within the cluster containing Priestia flexus MTCC 2909(T), Priestia aryabhattai B8W22(T) and Priestia megaterium KCTC 3007(T) and both strains showed highest similarity of > 98% with 3-29 nucleotide differences. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant isoprenoid quinone was MK-7 and the G + C content of strains was 37.5-37.7 mol%. However, the DNA-DNA hybridization and the phenotypic characteristics revealed that, the strain SGD-V-76(T) and strain SGD-M-37 are similar species but different from any known Priestia species with ANI values of 79.2, 79.3 and 79.2 and the dDDH values of 17.7, 17.8 and 18.0% respectively. On the basis of phenotypic characteristics, phylogenetic analysis and the results of biochemical and physiological tests, and genomic data strain SGD-V-76(T) was clearly distinguished from closely related members of the Priestia genus. Based on the above data analysis strain SGD-V-76(T) (= DSM28242(T) = KCTC33802(T) = CIP111056(T) = NCIM5510(T)) represents a novel species of the genus Priestia, and we propose the name Priestia veravalensis sp. nov.10aCoastal sediment10agenome10aPriestia10aVeraval1 aWagh, Vasudev, S.1 aRam, Hari1 aDastager, Syed, G.  uhttp://library.ncl.res.in/content/priestia-veravalensis-sp-nov-isolated-coastal-sample