02427nas a2200241 4500008004100000022001400041245015600055210006900211260000800280300000900288490000700297520153600304100002101840700002301861700001901884700002701903700002601930700002001956700002701976700002802003700002802031856012602059 2020 eng d a2045-232200aHighly sensitive label-free bio-interfacial colorimetric sensor based on silk fibroin-gold nanocomposite for facile detection of chlorpyrifos pesticide0 aHighly sensitive labelfree biointerfacial colorimetric sensor ba cMAR a41980 v103 a

Herein, the preparation of gold nanoparticles-silk fibroin (SF-AuNPs) dispersion and its label-free colorimetric detection of the organophosphate pesticide, namely chlorpyrifos, at ppb level are reported. The silk fibroin solution was extracted from B. mori silk after performing degumming, dissolving and dialysis steps. This fibroin solution was used for synthesis of gold nanoparticles in-situ without using any external reducing and capping agent. X-ray Diffractometry (XRD), Field Emission Transmission Electron Microscopy (FETEM) along with Surface Plasmon Resonance based optical evaluation confirmed generation of gold nanoparticles within SF matrix. The resultant SF-AuNPs dispersion exhibited rapid and excellent colorimetric pesticide sensing response even at 10 ppb concentration. Effect of additional parameters viz. pH, ionic concentration and interference from other pesticide samples was also studied. Notably, SF-AuNPs dispersion exhibited selective colorimetric pesticide sensing response which can be calibrated. Furthermore, this method was extended to various simulated real life samples such as tap water, soil and agricultural products including plant residues to successfully detect the presence of chlorpyrifos pesticide. The proposed colorimetric sensor system is facile yet effective and can be employed by novice rural population and expert researchers alike. It can be exploited as preliminary tool for label-free colorimetric chlorpyrifos pesticide sensing in water and agricultural products.

1 aMane, Pramod, C.1 aShinde, Manish, D.1 aVarma, Sanjana1 aChaudhari, Bhushan, P.1 aFatehmulla, Amanullah1 aShahabuddin, M.1 aAmalnerkar, Dinesh, P.1 aAldhafiri, Abdullah, M.1 aChaudhari, Ravindra, D. uhttp://library.ncl.res.in/content/highly-sensitive-label-free-bio-interfacial-colorimetric-sensor-based-silk-fibroin-gold02336nas a2200241 4500008004100000022001400041245015700055210006900212260000900281300001200290490000800302520146900310653001201779653001401791653002901805100001601834700002401850700002801874700001901902700002701921700002101948856012501969 2022 eng d a0141-813000aMechanistic insight into differential interactions of iron oxide nanoparticles with native, glycated albumin and their effect on erythrocytes parameters0 aMechanistic insight into differential interactions of iron oxide cJUL  a232-2470 v2123 a

Nanoparticles and protein bioconjugates have been studied for multiple biomedical applications. We sought to investigate the interaction and structural modifications of bovine serum albumin (BSA) with iron oxide nanoparticles (IONPs). The IONPs were green synthesized using E. crassipes aqueous leaf extract following characterization using transmission electron microscopy, energy dispersive X-ray analysis and X-ray diffraction. Two different concentrations of native/glycated albumin (0.5 and 1.5 mg/ml) with IONPs were allowed to interact for 1 h at 37 degrees C. Glycation markers, protein modification markers, cellular antioxidant, and hemolysis studies showed structural modifications and conformational changes in albumin due to the presence of IONPs. UV-visible absorbance resulted in hyperchromic and bathochromic effects of IONPs-BSA conjugates. Fluorescence measurements of tyrosine, tryptophan, advanced glycated end products, and ANS binding assay were promising and quenching effects proved IONPs-BSA conjugate formation. In FTIR of BSA-IONPs, transmittance was increased in amide A and B bands while decreased in amide I and II bands. In summary, native PAGE, HPLC, and FTIR analysis displayed a differential behaviour of IONPs with native and glycated BSA. These results provided an understanding of the interaction and structural modifications of glycated and native BSA which may provide fundamental repercussions in future studies.

10aAlbumin10aGlycation10airon oxide nanoparticles1 aNisar, Akib1 aAjabia, Devangi, K.1 aAgrawal, Sanskruthi, B.1 aVarma, Sanjana1 aChaudhari, Bhushan, P.1 aTupe, Rashmi, S. uhttp://library.ncl.res.in/content/mechanistic-insight-differential-interactions-iron-oxide-nanoparticles-native-glycated02395nas a2200205 4500008004100000020001400041245019500055210006900250260000800319520162100327100002101948700001901969700002301988700003002011700002302041700002802064700002302092700002702115856004702142 2022 eng d a2522-574X00aOne-pot concurrent biosynthesis of biphasic CuxO (cuprous and cupric oxide) nanoparticles using leaf extract of Eichhornia crassipes and investigation of their potent healthcare applications0 aOnepot concurrent biosynthesis of biphasic CuxO cuprous and cupr cFEB3 aEichhornia crassipes, an aquatic weed, has been used for the one-pot quick synthesis of cuprous and cupric oxide nanoparticles in this report. The identification and validation of the formed nanoparticles were successfully done by sophisticated characterization techniques such as UV–visible spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. Their biomedical interventions were assessed by their antioxidant and anticancer activities. The synthesized nanoparticles have a spherical morphology with an average diameter of 10 nm. Antioxidant assay performed showed an increasing trend in a dose-dependent manner with the percentage radical scavenging activity up to 94.70% at a much higher concentration of 1000 µg/ml. The antioxidant potential at such higher concentration is suspected to invoke a change in the cytotoxic potential of the nanoparticles which is then verified by the MTT assay. A significant cytotoxic activity against HeLa (cervical cancer) and HCT 116 (colorectal carcinoma) cell lines was detected with noted IC50 values of 17.17 and 13.70 µg/ml respectively. The conclusive findings obtained are enough to substantiate the use of these nanoparticles in biomedical areas specifically in anticancer treatment due to their high toxicities. The hazards imposed by Eichhornia crassipes can be alleviated by using them as biofactories for the synthesis of a variety of nanomaterials. This helps in curbing the water pollution issues as well as the developing synthesis protocols for robust and stable nanoparticles.1 aMoudgil, Aliesha1 aVarma, Sanjana1 aShinde, Manish, D.1 aVamkudoth, Koteswara, Rao1 aSarkar, Dhiman, M.1 aShende, Rajnigandha, A.1 aAmalnerkar, Dinesh1 aChaudhari, Bhushan, P. uhttps://doi.org/10.1007/s42247-022-00347-102872nas a2200277 4500008004100000022001400041245013300055210006900188260000800257300001200265490000700277520189600284653003802180653002302218653001702241653002302258653002702281100002102308700001902329700001702348700002502365700001802390700002702408700003002435856012902465 2023 eng d a1139-670900aBiosynthesis of gold nanoparticles by Penicillium rubens and catalytic detoxification of ochratoxin A and organic dye pollutants0 aBiosynthesis of gold nanoparticles by Penicillium rubens and cat cNOV a765-7800 v263 a

The environmental pollution caused by chemical dyes is a growing concern nowadays. Limitations of traditional methods opened the route for nanotechnology; owing to the versatile properties of nanomaterials, gold nanoparticles (AuNPs) became a potential strategy for different applications. In the present study, biosynthesis of gold nanoparticles (BioAuNPs) was carried out by reacting chloroauric acid (HAuCl4) with cell-free filtrate of Penicillium rubens sp. nov. NCIM 1937. The AuNPs were then characterized by UV-visible spectroscopy, HR-TEM, FTIR, and DLS analysis to further examine their efficacious biosynthesis and morphological properties including size, shape, and stability. The biogenic AuNPs are polydisperse in nature, with a mean size of 14.92 +/- 5 nm. These AuNPs exhibited promising antimicrobial activity against Escherichia coli NCIM-2065, Bacillus subtilis NCIM-2010, and Penicillium verrucosum MTCC 4935. In vitro quantitative HPLC results revealed that BioAuNPs significantly inhibited the biosynthesis of ochratoxin A (OTA). Microbial fuel cells (MFCs) are intriguing for power generation and wastewater treatment since they can directly transform chemical energy stored in organic matter to electricity by extracellular electron transfer (EET) via membrane proteins. AuNPs also showed excellent potential for dye degradation of organic pollutants, viz., methylene blue (MB), phenol red (PR), bromothymol blue (BTB), Congo red (CR), and 4-nitrophenol (4-NP). All dye removal efficiencies were estimated and fitted to pseudo-first-order processes using kinetic rate constants (Ka).The present study reveals a simple, original, and eco-friendly method for the synthesis of multifunctional biogenic AuNPs that could be effective in OTA detoxification in food products and organic pollutant removal during wastewater treatment for a sustainable environment.

10aCatalytic organic dye degradation10agold nanoparticles10aOchratoxin A10aPenicillium rubens10aPenicillium verrucosum1 aBhandari, Yogesh1 aVarma, Sanjana1 aSawant, Amol1 aBeemagani, Sreelatha1 aJaiswal, Neha1 aChaudhari, Bhushan, P.1 aVamkudoth, Koteswara, Rao uhttp://library.ncl.res.in/content/biosynthesis-gold-nanoparticles-penicillium-rubens-and-catalytic-detoxification-ochratoxin