02439nas a2200277   4500008004100000022001400041245013600055210006900191260000800260300001100268490000800279520148000287653001401767653001801781653003401799653002501833653001701858653002501875653001101900100002201911700002901933700002401962700002601986700002002012856012902032       2020                        eng d  a0926-669000aGeneration of novelties in the genus Ocimum as a result of natural hybridization: a morphological, genetical and chemical appraisal0 aGeneration of novelties in the genus Ocimum as a result of natur  cNOV  a1128590 v1563 a<p>The genus Ocimum is a boutique of a diverse set of specialized metabolites such as terpenoids and phenylpropanoids. Each Ocimum species and its cultivars represent a characteristic chemical profile. The present study explored the two interspecific Ocimum hybrids originating through a serendipitous natural cross between O. kilimandscharicum and O. basilicum. These two novel Ocimum hybrids exhibited intermediate morphological features of two parental species. Inter simple sequence repeats (ISSR) analysis and DNA barcoding with the plastid non-coding trnH-psbA intergenic spacer region reaffirmed unambiguous parental identification and differentiation of these natural hybrids from other available Ocimum species. Consequently, gas chromatographymass spectrometry-based metabolite profiling of two hybrids identified them as specific chemotypes with the presence of a unique blend of specialized metabolites from the parental species, which are either rich in terpenes or phenylpropanoids. Additionally, expression analysis of key genes from terpenoid and phenylpropanoid pathways corroborated with differential metabolite accumulation. Thus, these two Ocimum hybrids represented the novel chemotypes, which could be useful in commercial cultivation to produce novel essential oil and bioactive constituents. Further, the resulting metabolite diversity could have potential industrial applications in the areas of healthcare, bioremediation, and crop protection.</p>
10aChemotype10aDNA barcoding10aInter Simple Sequence Repeats10aInterspecific hybrid10aO. basilicum10aO. kilimandscharicum10aOcimum1 aGurav, Tanuja, P.1 aJayaramaiah, Ramesha, H.1 aPunekar, Sachin, A.1 aDholakia, Bhushan, B.1 aGiri, Ashok, P.  uhttp://library.ncl.res.in/content/generation-novelties-genus-ocimum-result-natural-hybridization-morphological-genetical-and