01558nas a2200217 4500008004100000022001400041245011500055210006900170260000800239300001400247490000700261520076500268100002701033700003001060700001601090700002701106700002301133700002801156700002501184856013101209 2019 eng d a1477-052000aDemystifying a hexuronic acid ligand that recognizes Toxoplasma gondii and blocks its invasion into host cells0 aDemystifying a hexuronic acid ligand that recognizes Toxoplasma cMAY a4535-45420 v173 a
Toxoplasma gondii is a ubiquitous eukaryotic pathogen responsible for toxoplasmosis in humans and animals. This parasite is an obligate intracellular pathogen and actively invades susceptible host cells, a process which is mediated by specific receptor-ligand interactions. Here, we have identified an unnatural 2,4-disulfated D-glucuronic acid (Di-S-GlcA), a hexuronic acid composed of heparin/ heparan sulfate, as a potential carbohydrate ligand that can selectively bind to T. gondii parasites. More importantly, the gelatin conjugated Di-S-GlcA multivalent probe displayed strong inhibition of parasite entry into host cells. These results open perspective for the future use of Di-S-GlcA epitopes in biomedical applications against toxoplasmosis.
1 aSubramani, Balamurugan1 aShantamurthy, Chethan, D.1 aMaru, Parag1 aBelekar, Meenakshi, A.1 aMardhekar, Sandhya1 aShanmugam, Dhanasekaran1 aKikkeri, Raghavendra uhttp://library.ncl.res.in/content/demystifying-hexuronic-acid-ligand-recognizes-toxoplasma-gondii-and-blocks-its-invasion-host