02382nas a2200205 4500008004100000245017400041210006900215260000800284300001400292490000700306520157700313100002301890700002301913700002001936700002601956700002001982700002602002700002202028856012602050 2017 eng d00aGlycoprotein alpha-amylase inhibitor from Withania somnifera differentially inhibits various alpha-amylases and affects the growth and development of Tribolium castaneum0 aGlycoprotein alphaamylase inhibitor from Withania somnifera diff cJUL a1382-13900 v733 a
BACKGROUND: Identification and characterisation of plant defensive molecules enrich our resources to design crop protection strategies. In particular, plant-derived proteinaceous inhibitor(s) of insect digestive enzymes appear to be a safe, sustainable and attractive option. RESULTS: A glycoprotein having non-competitive alpha-amylase inhibitory activity with a molecular weight of 8.3 kDa was isolated and purified from seeds of Withania somnifera alpha-amylase inhibitor (WSAI). Its mass spectrometry analysis revealed 59% sequence coverage with Wrightide II-type alpha-amylase inhibitor from Wrightia religiosa. A dose-dependent inhibition of alpha-amylases from Aspergillus oryzae, Bacillus subtilis, Helicoverpa armigera and Tribolium castaneumwas recorded. Interestingly, WSAI did not inhibit human salivary alpha-amylase significantly. When adults of T. castaneum were fed with WSAI (1.6mg g(-1)), decrease inconsumption, growthandefficiency of conversion of ingested foodwas evident, along withover fourfold increases in feedingdeterrence index. Adecline inlarval residual alpha-amylase activity after feedingofWSAI resulted ina reduction in longevity of T. castaneum. CONCLUSION: The study reflects the significance of WSAI in affecting the overall growth and development of T. castaneum. Pre-and post-harvest pest resistive capability makes WSAI a potential candidate for insect pest management. Further, the effectiveness of this inhibitor could be explored either in formulations or through a transgenic approach. (C) 2016 Society of Chemical Industry
1 aKasar, Sainath, S.1 aMarathe, Kiran, R.1 aBhide, Amey, J.1 aHerwade, Abhijeet, P.1 aGiri, Ashok, P.1 aMaheshwari, Vijay, L.1 aPawar, Pankaj, K. uhttp://library.ncl.res.in/content/glycoprotein-alpha-amylase-inhibitor-withania-somnifera-differentially-inhibits-various