01792nas a2200229 4500008004100000022001400041245006400055210006400119260006100183300001200244490000800256520104000264100001701304700002501321700002601346700001701372700002001389700002001409700002101429700001801450856009401468 2005 eng d a0040-575200aMolecular mapping of stem and leaf rust resistance in wheat0 aMolecular mapping of stem and leaf rust resistance in wheat a233 SPRING STREET, NEW YORK, NY 10013 USAbSPRINGERcSEP a846-8500 v1113 a
Stem rust caused by Puccinia graminis f. sp. tritici Eriks and Henn and leaf rust caused by Puccinia triticina Rob. ex Desm. are major constraints to wheat production worldwide. In the present study, F-4-derived SSD population, developed from a cross between Australian cultivars `Schomburgk' and `Yarralinka', was used to identify molecular markers linked to rust resistance genes Lr3a and Sr22. A total of 1,330 RAPD and 100 ISSR primers and 33 SSR primer pairs selected on the basis of chromosomal locations of these genes were used. The ISSR marker UBC 840(540) was found to be linked with Lr3a in repulsion at a distance of 6.0 cM. Markers cfa2019 and cfa2123 flanked Sr22 at a distance of 5.9 cM (distal) and 6.0 cM (proximal), respectively. The use of these markers in combination would predict the presence or absence of Sr22 in breeding populations. A previously identified PCR-based diagnostic marker STS638 linked to Lr20 was validated in this population. This marker showed a recombination value of 7.1 cM with Lr20.
1 aKhan, R., R.1 aBariana, Harbans, S.1 aDholakia, Bhushan, B.1 aNaik, S., V.1 aLagu, Meena, D.1 aRathjen, A., J.1 aBhavani, Sridhar1 aGupta, V., S. uhttp://library.ncl.res.in/content/molecular-mapping-stem-and-leaf-rust-resistance-wheat-0