01940nas a2200241 4500008004100000022001400041245015600055210006900211260007300280300001200353490000700365520100800372653002601380653003301406653002201439653001801461653001701479653001001496100002401506700002901530700001701559856012201576 2008 eng d a1369-703X00aIntegrated process for extraction and purification of alcohol dehydrogenase from Baker's yeast involving precipitation and aqueous two phase extraction0 aIntegrated process for extraction and purification of alcohol de aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAR a414-4200 v383 a
The potential of aqueous two phase extraction for the purification of alcohol dehydrogenase (ADH) from Baker's yeast (Saccharomyces cerevisiae) is demonstrated. An integrated process involving precipitation followed by aqueous two phase extraction (ATPE) is used for effective recovery of the enzyme. This is carried out in two schemes. In the first scheme, ammonium sulphate precipitation is carried out followed by ATPE of the precipitate after suspending it in buffer. While the second scheme involves polyethylene glycol precipitation followed by ATPE of the supernatant. The effect of various process parameters such as molecular weight of polymer, tie line length, phase volume ratio and neutral salt is investigated for enhancing the activity recovery of ADH. First scheme resulted in about 85% enzyme activity recovery of ADH with purification factor of 4.2 while the second scheme about 90% enzyme activity recovery with 6.6-fold purification. (C) 2007 Elsevier B.V. All rights reserved.
10aAlcohol dehydrogenase10aaqueous two phase extraction10aenzyme extraction10aPrecipitation10aPurification10aYeast1 aMadhusudhan, M., C.1 aRaghavarao, K., S. M. S.1 aNene, Sanjay uhttp://library.ncl.res.in/content/integrated-process-extraction-and-purification-alcohol-dehydrogenase-bakers-yeast-0