02437nas a2200241 4500008004100000022001400041245017900055210006900234260007600303300001200379490000800391520150800399653001901907653001601926653002101942653001001963653002401973653001901997100001702016700002102033700001902054856012202073 2008 eng d a1436-873000aAccumulation of cadmium in growing peanut (Arachis hypogaea L.) seedlings - its effect on lipid peroxidation and on the antioxidative enzymes catalase and gualacol peroxidase0 aAccumulation of cadmium in growing peanut Arachis hypogaea L see aCOMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USAbWILEY-BLACKWELLcJUN a440-4470 v1713 a
In plants exposed to high metal concentrations, mechanisms to counteract the oxidative burst are crucial for its survival. To investigate the temporal sequence of physiological reactions of peanut seedlings (Arachis hypogaea L.) to cadmium exposure, seeds were cultured in increasing concentrations of CdCl(2), ranging from 50 to 300 mu M. Germination frequency was scored, and the distributions of Cd in root, stem, and leaves were determined after 2 and 4 weeks of culture. Lipid peroxidation and activities of antioxidative enzymes including catalase (CAT, EC 1.11.1.6) and guaiacol peroxiclase (GPX; EC 1.11.1.7) were estimated in these three parts of the plant. Germination of seedlings was not affected, but the growth of seedlings was severely suppressed with increasing concentrations of CdCl(2) and incubation period. Pattern of Cd distribution in the three organs varied with concentration and period of exposure to Cd. Increased lipid peroxidation was detected in all parts of the developing seedlings with increasing metal accumulation. Catalase and guaiacol peroxidase activity varied in the three parts of the seedlings with concentration of Cd and incubation period. Guaiacol peroxidase activity appears to be more active in scavenging the reactive oxygen species in developing peanut seedlings. The results of the present experiment demonstrate the advantages of a tissue-culture model system in studying the complex network of interactions of various factors in stress tolerance.
10aAbiotic stress10aheavy metal10astress tolerance10aTBARS10athiobarbituric acid10aTissue culture1 aKumar, Sunil1 aMehta, Urmil, J.1 aHazra, Sulekha uhttp://library.ncl.res.in/content/accumulation-cadmium-growing-peanut-arachis-hypogaea-l-seedlings-its-effect-lipid-0