01851nas a2200241 4500008004100000022001400041245012100055210006900176260016500245300001200410490000700422520084600429653001601275653003601291653002001327653001501347653001001362100002401372700002501396700003701421700002501458856012601483 2011 eng d a0388-135000aComparative and chemical proteomic approaches reveal gatifloxacin deregulates enzymes involved in glucose metabolism0 aComparative and chemical proteomic approaches reveal gatifloxaci aINTERNATIONAL MEDICAL INFORMATION CENTER, SHINANOMACHI RENGAKAN, 35 SHINANO-MACHI, SHINJUKU-KU, TOKYO, 160-0016, JAPANbJAPANESE SOC TOXICOLOGICAL SCIENCEScDEC a787-7960 v363 a
Gatifloxacin has been associated with increased risks of hypoglycemic and hyperglycemic side effects. In order to understand the molecular mechanism of gatifloxacin induced deregulation of glucose metabolism, a Combination of comparative and chemical proteomic approaches were employed using yeast as a model system. Differential protein expression studies using two dimensional electrophoresis and mass spectrometry reveal that gatifloxacin deregulates the expression of key enzymes involved in glucose metabolism. Furthermore, affinity chromatography and LC-MSE analysis led to identification of enolase, as one of the key gatifloxacin binding proteins. Fluorescence spectrometric studies confirmed that the gatifloxacin indeed binds to enolase. Role of enolase in regulation of gatifloxacin induced dysglycemic effect is discussed.
10aantibiotics10aDifferential protein expression10aFluoroquinolone10aGlycolysis10aYeast1 aSuresh, Kesavan, K.1 aBhosale, Santosh, D.1 aThulasiram, Hirekodathakallu, V.1 aKulkarni, Mahesh, J. uhttp://library.ncl.res.in/content/comparative-and-chemical-proteomic-approaches-reveal-gatifloxacin-deregulates-enzymes-0