02527nas a2200241 4500008004100000022001400041245015000055210006900205260008000274300001200354490000700366520158700373653001901960653002201979653000802001653002602009653001802035653002002053100002902073700002302102700003102125856012902156 2013 eng d a1097-395800aMicrobial oxidation of medium chain fatty alcohol in the synthesis of sophorolipids by candida bombicola and its physicochemical characterization0 aMicrobial oxidation of medium chain fatty alcohol in the synthes aTIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANYbSPRINGER HEIDELBERGcMAR a173-1810 v163 a
Sophorolipids (SL) are glycolipid biosurfactants abundantly produced from different feedstocks by yeasts and have been widely developed for various applications. The amphipathic structures of sophorolipids imparts to them surfactant type properties. These biosurfactants are readily isolated in high yield and are nontoxic and biodegradable, which make them industrially interesting as surfactants or emulsifiers. Sophorolipid production was carried out using glucose as a hydrophilic source and lauryl alcohol C12-14, as a hydrophobic source by Candida bombicola (ATCC 22214). Process economics and environmental attractiveness was increased by using a low cost substrate. Optimization studies were carried out where the effect of glucose concentration, pH, temperature and metal ions on sophorolipid production was studied. Optimum production of sophorolipid obtained using lauryl alcohol (SLLA) was achieved after 96 h, the initial pH of the medium was 6.0 with 10 % glucose at 30 A degrees C. Primary characterization of SLLA was done by FTIR. The SLLA showed high physicochemical properties in terms of the surface activities when compared with synthetic surfactants including dodecyl tetraethylene glycol ether and sodium lauryl sulfate. The surface tension of SLLA thus obtained was found to be 24 mN/m with a critical micellar concentration (CMC) value of 0.68 mg/L. Antimicrobial testing showed that SLLA have potent antimicrobial activity against both gram negative bacteria, Escherichia coli (ATCC 8739) and gram positive cocci, Staphylococcus aureuss (ATCC 2079).
10aBiosurfactants10aCandida bombicola10aCMC10aLauryl alcohol C12-1410asophorolipids10aSurface tension1 aPulate, Vrushali, Dengle1 aBhagwat, Sunil, S.1 aPrabhune, Asmita, Ashutosh uhttp://library.ncl.res.in/content/microbial-oxidation-medium-chain-fatty-alcohol-synthesis-sophorolipids-candida-bombicola-0