01987nas a2200349 4500008004100000022001400041245009800055210006900153260008900222300001200311490000800323520087500331100001401206700001501220700001601235700002001251700001501271700002401286700002001310700001401330700002101344700001601365700001601381700001301397700001601410700001401426700001701440700001901457700002001476700001901496856012201515 2013 eng d a0036-807500aBiosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes0 aBiosynthesis of antinutritional alkaloids in solanaceous crops i a1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USAbAMER ASSOC ADVANCEMENT SCIENCEcJUL a175-1790 v3413 a
Steroidal glycoalkaloids (SGAs) such as alpha-solanine found in solanaceous food plants-as, for example, potato-are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.
1 aItkin, M.1 aHeinig, U.1 aTzfadia, O.1 aBhide, Amey, J.1 aShinde, B.1 aCardenas, Pablo, D.1 aBocobza, S., E.1 aUnger, T.1 aMalitsky, Sergey1 aFinkers, R.1 aTikunov, Y.1 aBovy, A.1 aChikate, Y.1 aSingh, P.1 aRogachev, I.1 aBeekwilder, J.1 aGiri, Ashok, P.1 aAharoni, Asaph uhttp://library.ncl.res.in/content/biosynthesis-antinutritional-alkaloids-solanaceous-crops-mediated-clustered-genes-0