01772nas a2200229 4500008004100000022001400041245014100055210006900196260007500265300001200340490000700352520086000359653002401219653004301243653001201286653003401298653002201332100001901354700002401373700002601397856011901423 2013 eng d a0006-292800aCloning and sequence analysis of three variants of the gene encoding alkaline xylanase C from the alkaliphilic bacillus sp (NCL 87-6-10)0 aCloning and sequence analysis of three variants of the gene enco a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGER/PLENUM PUBLISHERScOCT a737-7490 v513 a
Alkaline xylanase C from the alkaliphilic Bacillus sp. (NCL 87-6-10) has a low molecular weight and alkaline pI and is cellulase-free, properties compatible with its use in the prebleaching of pulp. We report here the cloning and sequence analysis of three variants of the gene encoding xylanase C; xyl C1, xyl C2, and xyl C3. In phylogenetic analysis, the three xylanase C variants clustered into a single group along with other reported alkaline xylanases. Residues contributing to the alkaline pH were present in all three variants. DNA and protein sequence comparison of these variants with other reported alkaline xylanases revealed silent mutations, some of which are due to codon preference in the respective organisms. The recombinant Xyl C1 that was successfully expressed in E. coli BL21 (DE3) had properties similar to the native enzyme.
10aAlkaline xylanase C10aAlkaliphilic Bacillus sp (NCL 87-6-10)10aCloning10aE. coli BL21 (DE3) expression10aSequence analysis1 aSharma, Poonam1 aRele, Meenakshi, V.1 aKumar, Lalitha, Sunil uhttp://library.ncl.res.in/content/cloning-and-sequence-analysis-three-variants-gene-encoding-alkaline-xylanase-c-0